13848
2015
eng
2843-2850
21
128
article
1
2016-09-19
--
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Prevalence of Nontraumatic Osteonecrosis of the Femoral Head and its Associated Risk Factors in the Chinese Population: Results from a Nationally Representative Survey
Background: Nontraumatic osteonecrosis of the femoral head (NONFH) is a debilitating disease that represents a significant financial burden for both individuals and healthcare systems. Despite its significance, however, its prevalence in the Chinese general population remains unknown. This study aimed to investigate the prevalence of NONFH and its associated risk factors in the Chinese population.
Methods: A nationally representative survey of 30,030 respondents was undertaken from June 2012 to August 2013. All participants underwent a questionnaire investigation, physical examination of hip, and bilateral hip joint X-ray and/or magnetic resonance imaging examination. Blood samples were taken after overnight fasting to test serum total cholesterol, triglyceride, and high-density lipoprotein (HDL) and low-density lipoprotein (LDL) levels. We then used multivariate logistic regression analysis to investigate the associations between various metabolic, demographic, and lifestyle-related variables and NONFH.
Results: NONFH was diagnosed in 218 subjects (0.725%) and the estimated NONFH cases were 8.12 million among Chinese people aged 15 years and over. The prevalence of NONFH was significantly higher in males than in females (1.02% vs. 0.51%, \(\chi^2\) = 24.997, P < 0.001). Among NONFH patients, North residents were subjected to higher prevalence of NONFH than that of South residents (0.85% vs. 0.61%, \(\chi^2\) = 5.847, P = 0.016). Our multivariate regression analysis showed that high blood levels of triglycerides, total cholesterol, LDL-cholesterol, and non-HDL-cholesterol, male, urban residence, family history of osteonecrosis of the femoral head, heavy smoking, alcohol abuse and glucocorticoid intake, overweight, and obesity were all significantly associated with an increased risk of NONFH.
Conclusions: Our findings highlight that NONFH is a significant public health challenge in China and underscore the need for policy measures on the national level. Furthermore, NONFH shares a number of risk factors with atherosclerosis.
Chinese Medical Journal
10.4103/0366-6999.168017
urn:nbn:de:bvb:20-opus-138482
Chinese Medical Journal 128, 21 (2015), pp. 2843-2850. doi:10.4103/0366-6999.168017
De-Wei Zhao
Mang Yu
Kai Hu
Wei Wang
Lei Yang
Ben-Jie Wang
Xiao-Hong Gao
Yong-Ming Guo
Yong-Qing Xu
Yu-Shan Wei
Si-Miao Tian
Fan Yang
Nan Wang
Shi-Bo Huang
Hui Xie
Xiao-Wei Wei
Hai-Shen Jiang
Yu-Qiang Zang
Jun Ai
Yuan-Liang Chen
Guang-Hua Lei
Yu-Jin Li
Geng Tian
Zong-Sheng Li
Yong Cao
Li Ma
eng
uncontrolled
nontraumatic osteonecrosis of the femoral head
eng
uncontrolled
risk factors
eng
uncontrolled
idiopathic osteonecrosis
eng
uncontrolled
early-stage osteonecrosis
eng
uncontrolled
implantation
eng
uncontrolled
bone
eng
uncontrolled
marrow
eng
uncontrolled
follow-up
eng
uncontrolled
intake
eng
uncontrolled
avascular necrosis
eng
uncontrolled
occupational-status
eng
uncontrolled
cigarette smoking
eng
uncontrolled
alcohol
eng
uncontrolled
prevalence
Medizin und Gesundheit
open_access
Medizinische Klinik und Poliklinik I
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/13848/ChineseMedJournal.pdf
23016
2018
eng
16
article
1
2021-03-09
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Wilms’ tumor 1-associating protein plays an aggressive role in diffuse large B-cell lymphoma and forms a complex with BCL6 via Hsp90
Background
Wilms’ tumor 1-associating protein (WTAP) is a nuclear protein, which is ubiquitously expressed in many tissues. Furthermore, in various types of malignancies WTAP is overexpressed and plays a role as an oncogene. The function of WTAP in diffuse large B-cell lymphoma (DLBCL), however, remains unclear.
Methods
Immunohistochemistry was applied to evaluate the levels of WTAP expression in DLBCL tissues and normal lymphoid tissues. Overexpression and knock-down of WTAP in DLBCL cell lines, verified on mRNA and protein level served to analyze cell proliferation and apoptosis in DLBCL cell lines by flow cytometry. Finally, co-immunoprecipitation (Co-IP), IP, and GST-pull down assessed the interaction of WTAP with Heat shock protein 90 (Hsp90) and B-cell lymphoma 6 (BCL6) as well as determined the extend of its ubiquitinylation.
Results
WTAP protein levels were consistently upregulated in DLBCL tissues. WTAP promoted DLBCL cell proliferation and improved the ability to confront apoptosis, while knockdown of WTAP in DLBCL cell lines allowed a significant higher apoptosis rate after treatment with Etoposide, an anti-tumor drug. The stable expression of WTAP was depended on Hsp90. In line, we demonstrated that WTAP could form a complex with BCL6 via Hsp90 in vivo and in vitro.
Conclusion
WTAP is highly expressed in DLBCL, promoting growth and anti-apoptosis in DLBCL cell lines. WTAP is a client protein of Hsp90 and can appear in a complex with BCL6 and Hsp90 in DLBCL. Down-regulation of WTAP could improve the chemotherapeutic treatments in DLBCL.
Cell Communication and Signaling
10.1186/s12964-018-0258-6
urn:nbn:de:bvb:20-opus-230168
publish
Cell Communication and Signaling (2018) 16:50. https://doi.org/10.1186/s12964-018-0258-6
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Yue Kuai
Xin Gong
Liya Ding
Fang Li
Lizhen Lei
Yuqi Gong
Qingmeng Liu
Huajiao Tan
Xinxia Zhang
Dongyu Liu
Guoping Ren
Hongyang Pan
Yaoyao Shi
Friederike Berberich-Siebelt
Zhengrong Ma
Ren Zhou
eng
uncontrolled
WTAP
eng
uncontrolled
BCL6
eng
uncontrolled
Hsp90
eng
uncontrolled
complex
eng
uncontrolled
DLBCL
Medizin und Gesundheit
open_access
Pathologisches Institut
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/23016/s12964-018-0258-6.pdf