7158
1993
eng
bookpart
1
2013-08-19
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An improved 32P-postlabelling assay for detection and quantitation of styrene 7,8-oxide-DNA adducts
Using DNA modified with [7-3H]styrene 7,8-oxide (SO) in vitro we have standardized the 32P-postlabelling assay for detecting SO-DNA adducts. Nuclease P 1-enriched adducts were 32P-labelled and purified by high-salt ( 4.0 M ammonium formate, pH 6.1} C1s reverse-phase TLC. After elution from the layer with 2-butoxyethanol:H20 (4:6), adducts were separated by two-dimensional PEI cellulose TLC in non-urea solvents (2.0 M ammonium formate, pH 3.5, and 2.7 M sodium phosphate, pH 5.6). One major, three minor and several trace adducts were detected. The efficiency of the kinase reaction depended on the ATP concentration. Use of standard labelling conditions (['Y· 32P]ATP, <3000 Ci/mmol; <2 Mikromol) resulted in poor ( 4-7%) adduct recovery. An ATP concentration of 40 Mikromol, however, increased the labeJling efficiency by a factor of 5-8 (35-55% based on 3H-SO labelied DNA). The results indicate that the new separation technique is suitable for the relatively polar SO-DNA adducts and that high labelling efficiency can be achieved.
7998
urn:nbn:de:bvb:20-opus-86305
In: Postlabelling methods for detection of DNA adducts / ed. by D. H. Phillips. - Lyon: IARC [u.a.], 1993. - (Centre International de Recherche sur le Cancer <Lyon>: IARC scientific publications ; 124). - S. 201-205
Deutsches Urheberrecht
S. Cantoreggi
R. C. Gupta
Werner K. Lutz
deu
swd
Medizin
Medizin und Gesundheit
open_access
Institut für Pharmakologie und Toxikologie
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7158/Lutz_7158.pdf
14984
2015
eng
339-346
3
6
article
1
2017-06-06
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Indications and selection of MR enterography vs. MR enteroclysis with emphasis on patients who need small bowel MRI and general anaesthesia: results of a survey
Aims
To survey the perceived indications for magnetic resonance imaging of the small bowel (MRE) by experts, when MR enteroclysis (MREc) or MR enterography (MREg) may be chosen, and to determine how the approach to MRE is modified when general anaesthesia (GA) is required.
Materials and methods
Selected opinion leaders in MRE completed a questionnaire that included clinical indications (MREg or MREc), specifics regarding administration of enteral contrast, and how the technique is altered to accommodate GA.
Results
Fourteen responded. Only the diagnosis and follow-up of Crohn’s disease were considered by over 80 % as a valid MRE indication. The remaining indications ranged between 35.7 % for diagnosis of caeliac disease and unknown sources of gastrointestinal bleeding to 78.6 % for motility disorders. The majority chose MREg over MREc for all indications (from 100 % for follow-up of caeliac disease to 57.7 % for tumour diagnosis). Fifty per cent of responders had needed to consider MRE under GA. The most commonly recommended procedural change was MRI without enteral distention. Three had experience with intubation under GA (MREc modification).
Conclusion
Views were variable. Requests for MRE under GA are not uncommon. Presently most opinion leaders suggest standard abdominal MRI when GA is required.
Insights into Imaging
10.1007/s13244-015-0384-2
PMC4444793
urn:nbn:de:bvb:20-opus-149847
Insights into Imaging (2015) 6:3, 339-346. DOI: 10.1007/s13244-015-0384-2
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Michael R. Torkzad
Gabriele Masselli
Steve Halligan
Aytek Oto
Henning Neubauer
Stuart Taylor
Arun Gupta
Jens Brøndum Frøkjær
Ian C. Lawrance
Christopher J. Welman
Anne Negård
Olle Ekberg
Michael Patak
Thomas Lauenstein
eng
uncontrolled
small bowel MRI
eng
uncontrolled
Crohn’s disease
eng
uncontrolled
general anaesthesia
Medizin und Gesundheit
open_access
Institut für diagnostische und interventionelle Radiologie (Institut für Röntgendiagnostik)
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/14984/098_Torkzad_Insights_into_Imaging.pdf