16594
2016
eng
13188
7
article
1
2018-07-27
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The fungal-specific beta-glucan-binding lectin FGB1 alters cell-wall composition and suppresses glucan-triggered immunity in plants
β-glucans are well-known modulators of the immune system in mammals but little is known about β-glucan triggered immunity in planta. Here we show by isothermal titration calorimetry, circular dichroism spectroscopy and nuclear magnetic resonance spectroscopy that the FGB1 gene from the root endophyte Piriformospora indica encodes for a secreted fungal-specific β-glucan-binding lectin with dual function. This lectin has the potential to both alter fungal cell wall composition and properties, and to efficiently suppress β-glucan-triggered immunity in different plant hosts, such as Arabidopsis, barley and Nicotiana benthamiana. Our results hint at the existence of fungal effectors that deregulate innate sensing of β-glucan in plants.
Nature Communications
10.1038/ncomms13188
urn:nbn:de:bvb:20-opus-165945
Nat Commun 7, 13188 (2016).
true
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Stephan Wawra
Philipp Fesel
Heidi Widmer
Malte Timm
Jürgen Seibel
Lisa Leson
Leona Kesseler
Robin Nostadt
Magdalena Hilbert
Gregor Langen
Alga Zuccaro
eng
uncontrolled
Effectors in plant pathology
eng
uncontrolled
Fungal host response
eng
uncontrolled
Lectins
Organische Chemie
open_access
Institut für Organische Chemie
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/16594/094_Wawra_NATURE_COMMUNICATIONS.pdf
6468
2012
eng
article
1
2013-03-12
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Goettingen Minipigs (GMP): Comparison of Two Different Models for Inducing Diabetes
Purpose: Preclinical experiments on large animals are indispensable for evaluating the effectiveness of diabetes therapies. Miniature swine are well suited for such studies due to their physiological and pathophysiological responses. Methods: We compare two methods for inducing diabetes in Goettingen minipigs (GMP), in five with the beta cell toxin streptozotocin (STZ) and in five other GMP by total pancreatectomy (PE). Glucose homeostasis was assessed with the intravenous glucose-tolerance test (IVGTT) and continual monitoring of interstitial glucose levels. At conclusion of the observation period, the pancreata were examined histologically. Three non-diabetic GMP served as control group. Results: The IVGTT revealed markedly diabetic profiles in both GMP groups. STZ-GMP were found to harbor residual C-peptides and scattered insulin-positive cells in the pancreas. PE-GMP survived the total pancreatectomy only with intensive postoperative care. Conclusions: Although both methods reliably induced diabetes in GMP, the PE-GMP clearly had more health problems and required a greater expenditure of time and resources. The PE-GMP model, however, was better at eliminating endogenous insulin and C-peptide than the STZ-GMP model.
urn:nbn:de:bvb:20-opus-75119
7511
In: Diabetology & Metabolic Syndrome (2012) 4: 7, doi:10.1186/1758-5996-4-7
Armin Strauss
Vasily Moskalenko
Christian Tiurbe
Irina Chodnevskaja
Stephan Timm
Verena A. Wiegering
Chrioph Thomas Germer
Karin Ulrichs
deu
swd
Göttingen
deu
swd
Minischwein
eng
uncontrolled
diabetes
eng
uncontrolled
pig or swine
eng
uncontrolled
real-time glucose monitoring
eng
uncontrolled
intravenous glucose tolerance test
eng
uncontrolled
total pancreatectomy
eng
uncontrolled
streptozotocin
Medizin und Gesundheit
open_access
Klinik und Poliklinik für Allgemein-, Viszeral-, Gefäß- und Kinderchirurgie (Chirurgische Klinik I)
Förderzeitraum 2012
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/6468/010_1758_5996_4_7.pdf