TY  - JOUR
A1  - Pinzner, Florian
A1  - Keller, Thorsten
A1  - Mut, Jürgen
A1  - Bechold, Julian
A1  - Seibel, Jürgen
A1  - Groll, Jürgen
T1  - Polyoxazolines with a vicinally double-bioactivated terminus for biomacromolecular affinity assessment
JF  - Sensors
N2  - Interactions between proteins and carbohydrates with larger biomacromolecules, e.g., lectins, are usually examined using self-assembled monolayers on target gold surfaces as a simplified model measuring setup. However, most of those measuring setups are either limited to a single substrate or do not allow for control over ligand distance and spacing. Here, we develop a synthetic strategy, consisting of a cascade of a thioesterification, native chemical ligation (NCL) and thiol-ene reaction, in order to create three-component polymer conjugates with a defined double bioactivation at the chain end. The target architecture is the vicinal attachment of two biomolecule residues to the α telechelic end point of a polymer and a thioether group at the ω chain end for fixating the conjugate to a gold sensor chip surface. As proof-of-principle studies for affinity measurements, we demonstrate the interaction between covalently bound mannose and ConA in surface acoustic wave (SAW) and surface plasmon resonance (SPR) experiments.
KW  - polyoxazolines
KW  - functionalization
KW  - lectin
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-239530
SN  - 1424-8220
VL  - 21
IS  - 9
ER  -