TY  - JOUR
A1  - Bassler, Miriam C.
A1  - Knoblich, Mona
A1  - Gerhard-Hartmann, Elena
A1  - Mukherjee, Ashutosh
A1  - Youssef, Almoatazbellah
A1  - Hagen, Rudolf
A1  - Haug, Lukas
A1  - Goncalves, Miguel
A1  - Scherzad, Agmal
A1  - Stöth, Manuel
A1  - Ostertag, Edwin
A1  - Steinke, Maria
A1  - Brecht, Marc
A1  - Hackenberg, Stephan
A1  - Meyer, Till Jasper
T1  - Differentiation of salivary gland and salivary gland tumor tissue via Raman imaging combined with multivariate data analysis
JF  - Diagnostics
N2  - Salivary gland tumors (SGTs) are a relevant, highly diverse subgroup of head and neck tumors whose entity determination can be difficult. Confocal Raman imaging in combination with multivariate data analysis may possibly support their correct classification. For the analysis of the translational potential of Raman imaging in SGT determination, a multi-stage evaluation process is necessary. By measuring a sample set of Warthin tumor, pleomorphic adenoma and non-tumor salivary gland tissue, Raman data were obtained and a thorough Raman band analysis was performed. This evaluation revealed highly overlapping Raman patterns with only minor spectral differences. Consequently, a principal component analysis (PCA) was calculated and further combined with a discriminant analysis (DA) to enable the best possible distinction. The PCA-DA model was characterized by accuracy, sensitivity, selectivity and precision values above 90% and validated by predicting model-unknown Raman spectra, of which 93% were classified correctly. Thus, we state our PCA-DA to be suitable for parotid tumor and non-salivary salivary gland tissue discrimination and prediction. For evaluation of the translational potential, further validation steps are necessary.
KW  - salivary gland tumor
KW  - confocal Raman imaging
KW  - principal component analysis
KW  - discriminant analysis
KW  - multivariate data analysis
KW  - molecular diagnostics
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-355558
SN  - 2075-4418
VL  - 14
IS  - 1
ER  -