TY  - JOUR
A1  - Ulrichs, Karin
A1  - Keller, R.
A1  - Müller-Ruchholtz, W.
T1  - Vorkommen und Manipulation von MHC Klasse II Antigenen auf Zellen isolierter Langerhans-Inseln
T1  - Presence and manipulation of MHC class II antigens on isolated island of Langerhans cells
N2  - No abstract available
KW  - Langerhans-Inseln
Y1  - 1986
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45499
ER  - 
TY  - JOUR
A1  - Heiser, A.
A1  - Ulrichs, Karin
A1  - Winoto-Morbach, S.
A1  - Vanucchi, A.
A1  - Jäger, H.
A1  - Müller-Ruchholtz, W.
T1  - Erste Kieler Erfahrungen mit 45 Isolierungen von Langerhansinseln aus Schweinepankreas
N2  - No abstract available.
KW  - Langerhans-Inseln
KW  - Bauchspeicheldrüse
KW  - Schwein
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-73205
ER  - 
TY  - JOUR
A1  - Heiser, A.
A1  - Ulrichs, Karin
A1  - Gier, C.
A1  - Müller-Ruchholtz, W.
T1  - Isolierung Langerhans'scher Inseln aus dem Pankreas von Schweinen: Einfluss biologischer und technischer Parameter auf die Inselausbeite und -integrität
N2  - No abstract available.
KW  - Langerhans-Inseln
KW  - Isolierung <Mikrobiologie>
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-73223
ER  - 
TY  - JOUR
A1  - Heiser, Axel
A1  - Ulrichs, Karin
A1  - Müller-Buchholtz, Wolfgang
T1  - Isolation of porcine pancreatic islets: low trypsin activity during the isolation procedure guarantees reproducible high islet yields
N2  - During the past few years, interest in xenotransplantation of porcine islets of Langerhans for the future therapy of type I diabetes has Increased markedly. Therefore, we established a semiautomated digestion method for isolating islets from the porcine pancreas. However, although the isolation technique was standardized and collagenase of controlled quality was used, we were unable to attain high islet yields with a satisfactory degree of reproducibility. One hypothesis was that varying degrees of interference by donor pancreatic enzymes were responsible for this failure. The aim of this stUdy was to examine the kinetics of four types of enzymatic activity during the isolation procedure, as well as their effects on islet yield: collagenase, trypsin, neutral protease, and clostripaln. Our results indicate that while exogenous collagenase activity decreases slightly during the isolation procedure, the activity of the pancreas enzymes neutral protease and trypsin increases. In some cases, trypsin activity increases very strongly. A strong increase in trypsin activity correlates with poor islet yield, whereas low trypsin activity always correlates with high islet yield. Addition of the protease inhibitor Pefabloc to the isolation medium results in low trypsin activity and reproducible high islet yields.
KW  - Langerhans-Inseln
KW  - islets of Langerhans
KW  - xenogeneic transplantation
KW  - swine
KW  - enzyme activation
KW  - enzyme inhibitors
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44719
ER  - 
TY  - JOUR
A1  - Heiser, A.
A1  - Müller-Ruchholtz, W.
A1  - Ulrichs, Karin
T1  - Isolation of Islets of Langerhans: Improvement of the Isolation Technique Using the Pig Model.
N2  - During the last view years, interest in pancreatic islet transplantation for the cure of type I diabetes has increased markedly. A serious barrier to clinical islet transplantation is the isolation of a sufficient mass of viable and functional islets. We used a porcine islet isolation model to examine various parameters of the isolation procedure and to improve isolation technique.
KW  - Langerhans-Inseln
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-87765
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Eckstein, V.
A1  - Tibell, A.
A1  - Groth, C. G.
A1  - Korsgren, O.
A1  - Müller-Ruchholtz, W.
A1  - Thiede, A.
T1  - Natürliche und induzierte xenoreaktive Antikörper vor und nach klinischer Transplantation fötaler porziner Pankreasinselzellen
N2  - No abstract available.
KW  - Antikörper
KW  - Langerhans-Inseln
KW  - Schwein
KW  - Transplantation
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-72967
ER  -