TY  - JOUR
A1  - Hickey, Scott F.
A1  - Sridhar, Malathy
A1  - Westermann, Alexander J.
A1  - Qin, Qian
A1  - Vijayendra, Pooja
A1  - Liou, Geoffrey
A1  - Hammond, Ming C.
T1  - Transgene regulation in plants by alternative splicing of a suicide exon
JF  - Nucleic Acids Research
N2  - Compared to transcriptional activation, other mechanisms of gene regulation have not been widely exploited for the control of transgenes. One barrier to the general use and application of alternative splicing is that splicing-regulated transgenes have not been shown to be reliably and simply designed. Here, we demonstrate that a cassette bearing a suicide exon can be inserted into a variety of open reading frames (ORFs), generating transgenes whose expression is activated by exon skipping in response to a specific protein inducer. The surprisingly minimal sequence requirements for the maintenance of splicing fidelity and regulation indicate that this splicing cassette can be used to regulate any ORF containing one of the amino acids Glu, Gln or Lys. Furthermore, a single copy of the splicing cassette was optimized by rational design to confer robust gene activation with no background expression in plants. Thus, conditional splicing has the potential to be generally useful for transgene regulation.
KW  - kingdom
KW  - pre-messenger RNA
KW  - gene expression
KW  - elements
KW  - decay
KW  - arabidopsis
KW  - eukaryotes
KW  - mechanisms
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-134724
VL  - 40
IS  - 10
ER  - 
TY  - JOUR
A1  - Seren, Ümit
A1  - Grimm, Dominik
A1  - Fitz, Joffrey
A1  - Weigel, Detlef
A1  - Nordborg, Magnus
A1  - Borgwardt, Karsten
A1  - Korte, Arthur
T1  - AraPheno: a public database for Arabidopsis thaliana phenotypes
JF  - Nucleic Acids Research
N2  - Natural genetic variation makes it possible to discover evolutionary changes that have been maintained in a population because they are advantageous. To understand genotype–phenotype relationships and to investigate trait architecture, the existence of both high-resolution genotypic and phenotypic data is necessary. Arabidopsis thaliana is a prime model for these purposes. This herb naturally occurs across much of the Eurasian continent and North America. Thus, it is exposed to a wide range of environmental factors and has been subject to natural selection under distinct conditions. Full genome sequencing data for more than 1000 different natural inbred lines are available, and this has encouraged the distributed generation of many types of phenotypic data. To leverage these data for meta analyses, AraPheno (https://arapheno.1001genomes.org) provide a central repository of population-scale phenotypes for A. thaliana inbred lines. AraPheno includes various features to easily access, download and visualize the phenotypic data. This will facilitate a comparative analysis of the many different types of phenotypic data, which is the base to further enhance our understanding of the genotype–phenotype map.
KW  - phenotype
KW  - arabidopsis
KW  - genotype
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-147909
VL  - 45
IS  - D1
ER  -