TY - JOUR A1 - Christl, Manfred A1 - Türk, M. A1 - Peters, K. A1 - Peters, E.-M. A1 - Schnering, H. G. von T1 - Octahydro-1,2,3:4,5,6-dimethenopentalen-2-carbonitril, das erste Derivat eines noch unbekannten (CH)\(_{10}\)-Kohlenwasserstoffs N2 - No abstract available KW - Organische Chemie Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58728 ER - TY - JOUR A1 - Schliephake, Andreas W. A1 - Rethwilm, Axel T1 - Nuclear Localization of Foamy Virus Gag Precursor Protein N2 - All foamy viruses give rise to a strong nuclear staining when infected cells are reacted with sera from infected hosts. This nuclear ftuorescence distinguishes foamy viruses from all other retroviruses. The experiments reported here indicate that the foamy virus Gag precursor protein is transiently located in the nuclei of infected cells and this is the likely reason for the typical foamy virus nuclear fluorescence. By using the vaccinia virus expression system, a conserved basic sequence motif in the nucleocapsid domain of foamy virus Cag proteins was identified to be responsible for the nuclear transport of the gag precursor molecule. Tbis motif was also found to be able to direct a heterologous protein, the Gag protein of human immunodeficiency virus, into the nucleus. KW - Virologie Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-61371 ER - TY - JOUR A1 - Hahn, Heidi A1 - Baunach, Gerald A1 - Bräutigam, Sandra A1 - Mergia, Ayalew A1 - Neumann-Haefelin, Dieter A1 - Daniel, Muthiah D. A1 - McClure, Myra O. A1 - Rethwilm, Axel T1 - Reactivity of primate sera to foamy virus Gag and Bet proteins N2 - In order to establish criteria for the Serodiagnosis of foamy virus infections we investigated the extent to which sera from iofected individuals of human and primate origin react with structural and non-structural virus proteins in immunoblot assays. Using lysates from infected cells as the source of virus antigen, antibodies were preferentially detected against the Gag proteins and the non-structural Bet protein. Both the Gag precursor molecules of 70 and 74K apparent M\(_r\) and the cytoplasmic 60K M\(_r\) Bet protein were found to be phosphorylated, the latter being synthesized in large amounts in infected cells. Rahbit antiserum raised against recombinant human foamy virus (HFV) Gag major capsid protein cross-reacted with foamy viruses of chimpanzee, gorilla, orang-utan, rhesus monkey and Mrican green monkey origin. This was reßected by a broad cross-reactivity of the respective monkey sera to the Gag proteins of the various foamy virus isolates. Cross-reactivity of antisera against the Bet protein was restricted to viruses from man and the great apes. Recombinant Gag and Bet proteins expressed in prokaryotes or in insect cells were readily recognized by foamy virus-positive primate sera. Screening serum samples from chimpanzees with HFV Gag and Bet proteins expressed by recombinant baculoviruses revealed that 18 out of 35 (52%) were positive for Gag antibodies. Of these, 13 (72 o/o) showed antiborlies against the Bet protein, indicating that Bet antigen is of value in sero1ogical screening for foamy virus infections. KW - Virologie Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-61366 ER - TY - JOUR A1 - Siwka, Wieslaw A1 - Schwinn, Andreas A1 - Baczko, Knut A1 - Pardowitz, Iancu A1 - Mhalu, Fred A1 - Shao, John A1 - Rethwilm, Axel A1 - ter Meulen, Volker T1 - vpu and env sequence variability of HIV-1 isolates from Tanzania N2 - No abstract available KW - Virologie Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-61355 ER - TY - JOUR A1 - Christl, Manfred A1 - Braun, Martin A1 - Wolz, E. A1 - Wagner, W. T1 - Cycloallene, 9 - 1-Phenyl-1-aza-3,4-cyclohexadien, das erste Isodihydropyridin: Erzeugung und Abfangreaktionen T1 - Cycloallenes, 9 - 1-Phenyl-1-aza-3,4-cyclohexadiene, the First Isodihydropyridine: Generation and Interception N2 - No abstract available KW - Organische Chemie KW - Isoquinolines KW - hexahydro- KW - Cyclobuta[c}pyridines KW - hexahydro- KW - Cycloadditions KW - [2 + 2]- and [4 + 2]- KW - 3-Azabicyclo{3 KW - 1 KW - 0]hexane KW - 6 KW - 6-dibromo-3-phenyl- KW - 2 KW - 4-Pentadienylamine KW - 3-n-butyl-N-phenyl- Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58714 ER - TY - JOUR A1 - Gerstner, E. A1 - Kemmer, R. A1 - Christl, Manfred T1 - Elektrophile Additionen an das Bicyclo[1.1.0]butan-System von Tricyclo[4.1.0.0\(^{2,7}\)]-heptan-Derivaten: Halogen-Elektrophile T1 - Electrophilic Additions to the Bicyclo[l.l.O)butane System of Tricyclo[4.1.0.0\(^{2,7}\)]heptane Derivatives: Halogen Electrophiles N2 - No abstract available KW - Organische Chemie KW - Norpinanes KW - preparation KW - Carbocations KW - classical and nonclassical KW - Neighbouring group participation KW - Halonium ions KW - Migratory aptitudes in carbocations Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58700 ER - TY - JOUR A1 - Christl, Manfred A1 - Gerstner, E. A1 - Kemmer, R. A1 - Llewellyn, G. A1 - Bentley, T. W. T1 - Elektrophile Additionen an das Bicyclo[1.1.0]butan-System von 1-Phenyl- und 1-(4-Anisyl)tricyclo[4.1.0.0\(^{2,7}\)]heptan: Säure-katalysierte Reaktionen mit Wasser und Methanol, Anlagerung von Essigsäure und Oxymercurierung N2 - No abstract available KW - Organische Chemie KW - 6-Norpinanols KW - 6-aryl- KW - preparation KW - 6-Norpinyl 3 KW - 5-dinitrobenzoates KW - hydrolysis KW - Carbocations KW - generation and rearrangement KW - 2-Norcaranols KW - 1-aryl- KW - Cyclohept-3-en-1-ols KW - 3-aryl- KW - conformation Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58696 ER - TY - JOUR A1 - Strik, Werner K. A1 - Dierks, Thomas A1 - Franzek, Ernst A1 - Stöber, Gerald A1 - Maurer, Konrad T1 - P 300 asymmetries in schizophrenia revisited with reference-independent methods N2 - Evidence of hemispheric asymmetries in schizophrenia has been reported from different research areas. Asymmetries in evoked potential P300 topography are still controversial because of inconsistent findings. In the present study. previous results of abnormal lateralization of P300 were replicated in stabilized residual Schizophrenie patients. Auditory P300 was recorded during an odd ball task in which subjeets detected rare target stimuli. Schizophrenie patients had the P300 peak shifted to the right hemisphere and differed signifieantly from age- and sex-matched normal control subjects who had left-lateralized P300 peaks. A comparison of different methods of assessment and analysis of the topographical features of the P300 electric fields showed that the extraction of reference-independent descriptors of P300 topography is a reliable and sensitive method for statistical handling of the maps. The results suggest left hemispheric dysfunction during cognitive tasks in a subgroup of Schizophrenie patients. Inconsistencies between previous sturlies are likely to be due to heterogeneous patient groups, which may have included patients in an acute Schizophrenie episode or patients in clinical remission. lnvestigation of the clinical meaning of P300 alterations requires careful psychopathological definition of the patient groups. KW - Schizophrenie KW - Laterality KW - evoked potentials KW - electroeneephalography Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63372 ER - TY - JOUR A1 - Lesch, K. P. A1 - Stöber, Gerald A1 - Balling, U. A1 - Franzek, Ernst A1 - Li, S. H. A1 - Ross, C. A. A1 - Newman, M. A1 - Beckmann, H. A1 - Riederer, P. T1 - Triplet repeats in clinical subtypes of schizophrenia: variation at the DRPLA (B37 CAG repeat) locus is not associated with periodic catatonia N2 - Clinical evidence for a dominant mode of inheritance and anticipation in periodic catatonia, a distinct subtype of schizophrenia, indicates that genes with triplet repeat expansions or other unstable repetitive elements affecting gene expression may be involved in the etiology of this disorder. Because patients affected with dentatorubral-pallidoluysian atrophy (DRPLA) may present with "schizophrenic" symptoms, we have investigated the DRPLA (B 37 CAG repeat) locus on chromosome 12 in 41 patients with periodic catatonia. The B 37 CAG repeat locus was highly polymorphic but all alleles in both the patient and control group had repeat sizes within the normal range. We conclude that variation at the DRPLA locus is unlikely to be associated with periodic catatonia. The evidence for dominant inheritance and anticipation as well as the high prevalence of human brain genes containing trinucleotide repeats justifies further screening for triplet repeat expansions in periodic catatonia. KW - Schizophrenie KW - Association study KW - B 37 CAG repeat locus KW - chromosome 12 KW - schizophrenia KW - periodic catatonia Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63369 ER - TY - JOUR A1 - Strik, Werner K. A1 - Dierks, Thomas A1 - Franzek, Ernst A1 - Stöber, Gerald A1 - Maurer, Konrad T1 - P300 in Schizophrenia: Interactions between Amplitudes and Topography N2 - Low P300 amplitudes and topographical asymmetries have been reponed in schizophrenic patients, but reference-independent amplitude assessment failed to replicate reduced amplitudes. P300 amplitude is conventially assessed at midline electrodes (PZ), anti asymmetric topography as reported in schizophrenics, may conj'ound this measurement. We lnvestigated the possible Interaction between P300 ropography and assessments of amplitudes. ln 41 clinically stable schizophrenics and 31 normal controls, the generalfinding ofreduced amplitudes at the P'l electrode and topographical asymmetrles in the patient group were replicated. ln both groups, a.symmetries of the P300 field (lateralized peaks) reduced the standard amplitude assessment at the midline parletal electrode, but did not Qjfoct the reference-independent, global amplitude assessment. This shows thal asymmetry per se does not imply reduced field strength. in addition, in schizophreraics. but not in controls, there was a significcmt effect oftlae direction of asymmetry on both amplltude measures, amplitudes belng lower with increasing shift ofthe P300 peak to the right side. Considering also the slightly left-lateralized peaks in the normal controls. this suggests rhat only right lateralized P300 peaks upressfunctional deficits in schizophrenics, whereas left lateralized pealcs fall wlthin the physiological variability of the P3OO field. Tht refonnce-independent amplitude assessment is proposed for unambiguous amplitude assessment in order to better define the clinical, psychological and physiopathological mtaning of the P3OO alterations in schizophrenics. KW - Schizophrenie KW - Event-related potentials KW - P300 KW - P300 topography KW - Brain mappins KW - Schizophrenia Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63351 ER -