TY  - THES
A1  - von Wardenburg, Niels Oliver
T1  - Investigations into the Pathogenic Antibody-Antigen-Interference of Glycine Receptor Autoantibodies
T1  - Untersuchungen zur pathogenen Antikörper-Antigen-Interferenz von Autoantikörpern gegen den Glycinrezeptor
N2  - Anti-glycine receptor (GlyR) autoantibodies belong to the novel group of autoantibodies that target neuronal cell-surface antigens (NCS), which are accompanied with various neurologic and neuropsychiatric conditions. The inhibitory ionotropic GlyR is one of the major inhibitory neurotransmitter receptors and therefore involved in maintaining homeostasis of neuronal excitation levels at brain stem and spinal cord. Anti-GlyR autoantibodies are associated with progressive encephalomyelitis with rigidity and myoclonus or stiff person syndrome. These neuromotor disorders are characterized by exaggerated startle, muscle stiffness, and painful spasms, leading to immobility and fatal outcome in some cases. It was hypothesized that imbalance of motoneuronal inhibition by functional impairment of GlyR and receptor internalization are direct consequences of antibody-antigen interference. Here, serum samples of four patients were tested for anti-GlyR autoantibodies and were used for the analysis of the functional impact on the electrophysiological properties of recombinant GlyRs, transiently expressed in HEK293 cells. Furthermore, the recognition pattern of anti- GlyR autoantibodies to human, zebrafish and chimeric GlyRα1 located the epitope to the far N-terminal region. The pathogenicity of anti-GlyR autoantibodies and thereby the autoimmunologic etiology of the disease was confirmed by passive transfer of patient serum to zebrafish (Danio rerio) larvae, that yielded an abnormal escape response – a brain stem reflex that corresponds to the exaggerated startle of afflicted patients. The phenotype was accompanied by profound reduction of GlyR clusters in spinal cord cryosections of treated zebrafish larvae. Together, these novel insights into the pathogenicity of GlyR autoantibodies confirm the concept of a novel neurologic autoimmune disease and might contribute to the development of innovative therapeutic strategies.
N2  - GlyR Autoantikörper sind mit dem Stiff-Person-Syndrom assoziiert, insbesondere mit der schwerverlaufenden Variante der Progressiven Enzephalopathie mit Rigidität und Myoklonus. Diese Studie hat sich als Ziel gesetzt, die Pathogenität der Autoantikörper sowie deren pathogenen Eigenschaften mit Hilfe der Patch-Clamp-Methode sowie eines passiven Transfers der Erkrankung auf Zebrafischlarven zu erklären. ...
KW  - stiff person syndrome
KW  - glycine receptor autoantibodies
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-247217
ER  -