TY  - JOUR
A1  - Meier, Doreen
A1  - Kruse, Janis
A1  - Buttlar, Jann
A1  - Friedrich, Michael
A1  - Zenk, Fides
A1  - Boesler, Benjamin
A1  - Forstner, Konrad U.
A1  - Hammann, Christian
A1  - Nellen, Wolfgang
T1  - Analysis of the Microprocessor in Dictyostelium: The Role of RbdB, a dsRNA Binding Protein
JF  - PLoS Genetics
N2  - We identified the dsRNA binding protein RbdB as an essential component in miRNA processing in Dictyostelium discoideum. RbdB is a nuclear protein that accumulates, together with Dicer B, in nucleolar foci reminiscent of plant dicing bodies. Disruption of rbdB results in loss of miRNAs and accumulation of primary miRNAs. The phenotype can be rescued by ectopic expression of RbdB thus allowing for a detailed analysis of domain function. The lack of cytoplasmic dsRBD proteins involved in miRNA processing, suggests that both processing steps take place in the nucleus thus resembling the plant pathway. However, we also find features e.g. in the domain structure of Dicer which suggest similarities to animals. Reduction of miRNAs in the rbdB- strain and their increase in the Argonaute A knock out allowed the definition of new miRNAs one of which appears to belong to a new non-canonical class.
KW  - microprocessor
KW  - Dictyostelium discoideum
KW  - dsRNA binding protein
KW  - RbdB
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-166687
VL  - 12
IS  - 6
ER  -