TY  - JOUR
A1  - Abdelmohsen, Usama Ramadan
A1  - Yang, Chen
A1  - Horn, Hannes
A1  - Hajjar, Dina
A1  - Ravasi, Timothy
A1  - Hentschel, Ute
T1  - Actinomycetes from Red Sea Sponges: Sources for Chemical and Phylogenetic Diversity
N2  - The diversity of actinomycetes associated with marine sponges collected off Fsar Reef (Saudi Arabia) was investigated in the present study. Forty-seven actinomycetes were cultivated and phylogenetically identified based on 16S rRNA gene sequencing and were assigned to 10 different actinomycete genera. Eight putatively novel species belonging to genera Kocuria, Mycobacterium, Nocardia, and Rhodococcus were identified based on sequence similarity values below 98.2% to other 16S rRNA gene sequences available in the NCBI database. PCR-based screening for biosynthetic genes including type I and type II polyketide synthases (PKS-I, PKS-II) as well as nonribosomal peptide synthetases (NRPS) showed that 20 actinomycete isolates encoded each at least one type of biosynthetic gene. The organic extracts of nine isolates displayed bioactivity against at least one of the test pathogens, which were Gram-positive and Gram-negative bacteria, fungi, human parasites, as well as in a West Nile Virus protease enzymatic assay. These results emphasize that marine sponges are a prolific resource for novel bioactive actinomycetes with potential for drug discovery.
KW  - PKS I
KW  - Meeresschwämme
KW  - PKS II
KW  - NRPS
KW  - Red sea
KW  - sponges
KW  - actinomycetes
KW  - bioactivity
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-112882
ER  - 
TY  - JOUR
A1  - Hentschel, Ute
A1  - Kamke, Janine
A1  - Rinke, Christian
A1  - Schwientek, Patrick
A1  - Mavromatis, Kostas Mavromatis
A1  - Ivanova, Natalia
A1  - Sczyrba, Alexander
A1  - Woyke, Tanja
T1  - The Candidate Phylum Poribacteria by Single-Cell Genomics: New Insights into Phylogeny, Cell-Compartmentation, Eukaryote-Like Repeat Proteins, and Other Genomic Features
N2  - The candidate phylum Poribacteria is one of the most dominant and widespread members of the microbial communities residing within marine sponges. Cell compartmentalization had been postulated along with their discovery about a decade ago and their phylogenetic association to the Planctomycetes, Verrucomicrobia, Chlamydiae superphylum was proposed soon thereafter. In the present study we revised these features based on genomic data obtained from six poribacterial single cells. We propose that Poribacteria form a distinct monophyletic phylum contiguous to the PVC superphylum together with other candidate phyla. Our genomic analyses supported the possibility of cell compartmentalization in form of bacterial microcompartments. Further analyses of eukaryote-like protein domains stressed the importance of such proteins with features including tetratricopeptide repeats, leucin rich repeats as well as low density lipoproteins receptor repeats, the latter of which are reported here for the first time from a sponge symbiont. Finally, examining the most abundant protein domain family on poribacterial genomes revealed diverse phyH family proteins, some of which may be related to dissolved organic posphorus uptake.
KW  - Candidate Phylum Poribacteria
KW  - protein domains
KW  - genomic databases
KW  - phylogenetic analysis
KW  - genome analysis
KW  - sponges
KW  - marine bacteria
KW  - phylogenetics
KW  - polyvinyl chloride
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-112649
N1  - This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
ER  -