TY - JOUR A1 - Meyer, Malin Tordis A1 - Watermann, Christoph A1 - Dreyer, Thomas A1 - Wagner, Steffen A1 - Wittekindt, Claus A1 - Klussmann, Jens Peter A1 - Ergün, Süleyman A1 - Baumgart-Vogt, Eveline A1 - Karnati, Srikanth T1 - Differential expression of peroxisomal proteins in distinct types of parotid gland tumors JF - International Journal of Molecular Sciences N2 - Salivary gland cancers are rare but aggressive tumors that have poor prognosis and lack effective cure. Of those, parotid tumors constitute the majority. Functioning as metabolic machinery contributing to cellular redox balance, peroxisomes have emerged as crucial players in tumorigenesis. Studies on murine and human cells have examined the role of peroxisomes in carcinogenesis with conflicting results. These studies either examined the consequences of altered peroxisomal proliferators or compared their expression in healthy and neoplastic tissues. None, however, examined such differences exclusively in human parotid tissue or extended comparison to peroxisomal proteins and their associated gene expressions. Therefore, we examined differences in peroxisomal dynamics in parotid tumors of different morphologies. Using immunofluorescence and quantitative PCR, we compared the expression levels of key peroxisomal enzymes and proliferators in healthy and neoplastic parotid tissue samples. Three parotid tumor subtypes were examined: pleomorphic adenoma, mucoepidermoid carcinoma and acinic cell carcinoma. We observed higher expression of peroxisomal matrix proteins in neoplastic samples with exceptional down regulation of certain enzymes; however, the degree of expression varied between tumor subtypes. Our findings confirm previous experimental results on other organ tissues and suggest peroxisomes as possible therapeutic targets or markers in all or certain subtypes of parotid neoplasms. KW - peroxisomes KW - parotid gland KW - salivary KW - tumors KW - pleomorphic adenoma KW - mucoepidermoid carcinoma KW - acinic cell carcinoma KW - differential expression KW - immunohistochemistry KW - mRNA Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-261047 SN - 1422-0067 VL - 22 IS - 15 ER - TY - THES A1 - Groth, Sofie Claire T1 - Korrelation der Elastizität von Rückenmarksgewebe und histologischen Veränderungen in einem Tiermodell der Multiplen Sklerose T1 - Correlation of elasticity of spinal cord tissues and histological changes in an animal model of multiple sclerosis N2 - Multiple Sklerose ist eine der häufigsten und bedeutsamsten entzündlichen Autoimmunerkrankungen bei jungen Erwachsenen. Obwohl die klassischen Kennzeichen der Krankheit wie Infiltration von Immunzellen, Demyelinisierung, Astrogliose und axonale Schädigung bekannt sind, sind die genauen Ursachen und die zugrundeliegende Pathophysiologie noch nicht geklärt. In der Fachliteratur wurden bereits biomechanische Veränderungen mit histologischen Veränderungen im ZNS in Verbindung gebracht. Der genaue Zusammenhang und das Ausmaß zwischen den mechanischen Gewebeeigenschaften und den zugrundeliegenden histologischen Veränderungen wurde bis heute jedoch nur wenig erforscht. Die vorliegende Arbeit untersuchte in ihrem methodischen Rahmen den möglichen Zusammenhang zwischen den mechanischen Veränderungen des Gewebes und den zugrundeliegenden histologischen Gewebeveränderungen in den unterschiedlichen Krankheitsstadien der EAE, dem Tiermodell der MS. Die hier dargestellten Experimente konnten demonstrieren, dass das ZNS-Gewebe durch zunehmende Zelldichte steifer wird, während es bei fortschreitender Demyelinisierung zur Erweichung des Gewebes kommt. Ferner wurden die mechanischen Gewebeeigenschaften in den unterschiedlichen Krankheitsstadien der EAE durch die Astrogliose und die Mikroglia/Makrophageninfiltration beeinflusst. N2 - Multiple sclerosis is one of the most frequent and significant autoimmune inflammatory diseases in young adults. Although the classic hallmarks of the disease, such as immune cell infiltration, demyelination, gliosis and axonal damage, are known, the causes and underlying pathophysiology remain largely elusive. In recent studies biomechanical changes have already been associated with histological changes in the CNS. However, the correlation between tissue stiffness and the underlying structural changes is currently poorly understood. In this thesis I investigated how tissue stiffness is linked to the underlying structural changes during the different stages of an experimental autoimmune encephalomyelitis mouse model of MS. My data indicate that an increase in cell density leads to an increase in the CNS tissue stiffness, while demyelination reduces tissue stiffness. Furthermore, the mechanical properties were influenced by gliosis and microglia / macrophage infiltration. KW - Multiple Sklerose KW - Experimentelle autoimmune Enzepahlomyelitis KW - Biomechanische Eigenschaften KW - Immunhistochemie KW - Multiple sclerosis KW - Experimental autoimmune encephalomyelitis KW - Biomechanical properties KW - immunohistochemistry Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-179370 ER -