TY  - JOUR
A1  - Dashti, Yousef
A1  - Grkovic, Tanja
A1  - Abdelmohsen, Usama Ramadan
A1  - Hentschel, Ute
A1  - Quinn, Ronald J.
T1  - Production of Induced Secondary Metabolites by a Co-Culture of Sponge-Associated Actinomycetes, Actinokineospora sp EG49 and Nocardiopsis sp RV163
JF  - MARINE DRUGS
N2  - Two sponge-derived actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163, were grown in co-culture and the presence of induced metabolites monitored by H-1 NMR. Ten known compounds, including angucycline, diketopiperazine and beta-carboline derivatives 1-10, were isolated from the EtOAc extracts of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Co-cultivation of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 induced the biosynthesis of three natural products that were not detected in the single culture of either microorganism, namely N-(2-hydroxyphenyl)-acetamide (11), 1,6-dihydroxyphenazine (12) and 5a, 6,11a, 12-tetrahydro-5a, 11a-dimethyl[1,4]benzoxazino[3,2-b][1,4]benzoxazine (13a). When tested for biological activity against a range of bacteria and parasites, only the phenazine 12 was active against Bacillus sp. P25, Trypanosoma brucei and interestingly, against Actinokineospora sp. EG49. These findings highlight the co-cultivation approach as an effective strategy to access the bioactive secondary metabolites hidden in the genomes of marine actinomycetes.
KW  - co-cultivation
KW  - induced metabolites
KW  - sponge-associated actinomyetes
KW  - NMR fingerprint
KW  - bioactivity
KW  - natural products
KW  - A-D
KW  - aspergillus fumigatus
KW  - marine
KW  - biosynthesis
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-116547
SN  - 1660-3397
VL  - 12
IS  - 5
ER  -