TY  - JOUR
A1  - Binas, Oliver
A1  - Bessi, Irene
A1  - Schwalbe, Harald
T1  - Structure Validation of G‐Rich RNAs in Noncoding Regions of the Human Genome
JF  - ChemBioChem
N2  - We present the rapid biophysical characterization of six previously reported putative G‐quadruplex‐forming RNAs from the 5′‐untranslated region (5′‐UTR) of silvestrol‐sensitive transcripts for investigation of their secondary structures. By NMR and CD spectroscopic analysis, we found that only a single sequence—[AGG]\(_{2}\)[CGG]\(_{2}\)C—folds into a single well‐defined G‐quadruplex structure. Sequences with longer poly‐G strands form unspecific aggregates, whereas CGG‐repeat‐containing sequences exhibit a temperature‐dependent equilibrium between a hairpin and a G‐quadruplex structure. The applied experimental strategy is fast and provides robust readout for G‐quadruplex‐forming capacities of RNA oligomers.
KW  - biophysical investigation
KW  - circular dichroism
KW  - G-quadruplexes
KW  - NMR spectroscopy
KW  - RNA
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-214892
VL  - 21
IS  - 11
SP  - 1656
EP  - 1663
ER  -