TY  - JOUR
A1  - Veepaschit, Jyotishman
A1  - Viswanathan, Aravindan
A1  - Bordonne, Remy
A1  - Grimm, Clemens
A1  - Fischer, Utz
T1  - Identification and structural analysis of the Schizosaccharomyces pombe SMN complex
JF  - Nucleic Acids Research
N2  - The macromolecular SMN complex facilitates the formation of Sm-class ribonucleoproteins involved in mRNA processing (UsnRNPs). While biochemical studies have revealed key activities of the SMN complex, its structural investigation is lagging behind. Here we report on the identification and structural determination of the SMN complex from the lower eukaryote Schizosaccharomyces pombe, consisting of SMN, Gemin2, 6, 7, 8 and Sm proteins. The core of the SMN complex is formed by several copies of SMN tethered through its C-terminal alpha-helices arranged with alternating polarity. This creates a central platform onto which Gemin8 binds and recruits Gemins 6 and 7. The N-terminal parts of the SMN molecules extrude via flexible linkers from the core and enable binding of Gemin2 and Sm proteins. Our data identify the SMN complex as a multivalent hub where Sm proteins are collected in its periphery to allow their joining with UsnRNA.
KW  - Schizosaccharomyces pombe
KW  - SMN
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-259880
VL  - 49
IS  - 13
ER  - 
TY  - JOUR
A1  - Vendelova, Emilia
A1  - de Lima, Jeferson Camargo
A1  - Lorenzatto, Karina Rodrigues
A1  - Monteiro, Karina Mariante
A1  - Mueller, Thomas
A1  - Veepaschit, Jyotishman
A1  - Grimm, Clemens
A1  - Brehm, Klaus
A1  - Hrčková, Gabriela
A1  - Lutz, Manfred B.
A1  - Ferreira, Henrique B.
A1  - Nono, Justin Komguep
T1  - Proteomic Analysis of Excretory-Secretory Products of Mesocestoides corti Metacestodes Reveals Potential Suppressors of Dendritic Cell Functions
JF  - PLoS Neglected Tropical Diseases
N2  - Accumulating evidences have assigned a central role to parasite-derived proteins in immunomodulation. Here, we report on the proteomic identification and characterization of immunomodulatory excretory-secretory (ES) products from the metacestode larva (tetrathyridium) of the tapeworm Mesocestoides corti (syn. M. vogae). We demonstrate that ES products but not larval homogenates inhibit the stimuli-driven release of the pro-inflammatory, Th1-inducing cytokine IL-12p70 by murine bone marrow-derived dendritic cells (BMDCs). Within the ES fraction, we biochemically narrowed down the immunosuppressive activity to glycoproteins since active components were lipid-free, but sensitive to heat- and carbohydrate-treatment. Finally, using bioassay-guided chromatographic analyses assisted by comparative proteomics of active and inactive fractions of the ES products, we defined a comprehensive list of candidate proteins released by M. corti tetrathyridia as potential suppressors of DC functions. Our study provides a comprehensive library of somatic and ES products and highlight some candidate parasite factors that might drive the subversion of DC functions to facilitate the persistence of M. corti tetrathyridia in their hosts.
KW  - proteomic analysis
KW  - excretory-secretory
KW  - Mesocestoides corti
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-166742
VL  - 10
IS  - 10
ER  -