TY  - JOUR
A1  - Heiling, Sven
A1  - Knutti, Nadine
A1  - Scherr, Franziska
A1  - Geiger, Jörg
A1  - Weikert, Juliane
A1  - Rose, Michael
A1  - Jahns, Roland
A1  - Ceglarek, Uta
A1  - Scherag, André
A1  - Kiehntopf, Michael
T1  - Metabolite ratios as quality indicators for pre-analytical variation in serum and EDTA plasma
JF  - Metabolites
N2  - In clinical diagnostics and research, blood samples are one of the most frequently used materials. Nevertheless, exploring the chemical composition of human plasma and serum is challenging due to the highly dynamic influence of pre-analytical variation. A prominent example is the variability in pre-centrifugation delay (time-to-centrifugation; TTC). Quality indicators (QI) reflecting sample TTC are of utmost importance in assessing sample history and resulting sample quality, which is essential for accurate diagnostics and conclusive, reproducible research. In the present study, we subjected human blood to varying TTCs at room temperature prior to processing for plasma or serum preparation. Potential sample QIs were identified by Ultra high pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) based metabolite profiling in samples from healthy volunteers (n = 10). Selected QIs were validated by a targeted MS/MS approach in two independent sets of samples from patients (n = 40 and n = 70). In serum, the hypoxanthine/guanosine (HG) and hypoxanthine/inosine (HI) ratios demonstrated high diagnostic performance (Sensitivity/Specificity > 80%) for the discrimination of samples with a TTC > 1 h. We identified several eicosanoids, such as 12-HETE, 15-(S)-HETE, 8-(S)-HETE, 12-oxo-HETE, (±)13-HODE and 12-(S)-HEPE as QIs for a pre-centrifugation delay > 2 h. 12-HETE, 12-oxo-HETE, 8-(S)-HETE, and 12-(S)-HEPE, and the HI- and HG-ratios could be validated in patient samples.
KW  - quality indicators
KW  - biomarker
KW  - hypoxanthine
KW  - inosine
KW  - guanosine
KW  - eicosanoids
KW  - time-to-centrifugation
KW  - pre-analytical variation
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-246261
SN  - 2218-1989
VL  - 11
IS  - 9
ER  -