TY  - JOUR
A1  - Counsell, John R.
A1  - Karda, Rajvinder
A1  - Diaz, Juan Antiano
A1  - Carey, Louise
A1  - Wiktorowicz, Tatiana
A1  - Buckley, Suzanne M. K.
A1  - Ameri, Shima
A1  - Ng, Joanne
A1  - Baruteau, Julien
A1  - Almeida, Filipa
A1  - de Silva, Rohan
A1  - Simone, Roberto
A1  - Lugarà, Eleonora
A1  - Lignani, Gabriele
A1  - Lindemann, Dirk
A1  - Rethwilm, Axel
A1  - Rahim, Ahad A.
A1  - Waddington, Simon N.
A1  - Howe, Steven J.
T1  - Foamy Virus Vectors Transduce Visceral Organs and Hippocampal Structures following In Vivo Delivery to Neonatal Mice
JF  - Molecular Therapy: Nucleic Acids
N2  - Viral vectors are rapidly being developed for a range of applications in research and gene therapy. Prototype foamy virus (PFV) vectors have been described for gene therapy, although their use has mainly been restricted to ex vivo stem cell modification. Here we report direct in vivo transgene delivery with PFV vectors carrying reporter gene constructs. In our investigations, systemic PFV vector delivery to neonatal mice gave transgene expression in the heart, xiphisternum, liver, pancreas, and gut, whereas intracranial administration produced brain expression until animals were euthanized 49 days post-transduction. Immunostaining and confocal microscopy analysis of injected brains showed that transgene expression was highly localized to hippocampal architecture despite vector delivery being administered to the lateral ventricle. This was compared with intracranial biodistribution of lentiviral vectors and adeno-associated virus vectors, which gave a broad, non-specific spread through the neonatal mouse brain without regional localization, even when administered at lower copy numbers. Our work demonstrates that PFV can be used for neonatal gene delivery with an intracranial expression profile that localizes to hippocampal neurons, potentially because of the mitotic status of the targeted cells, which could be of use for research applications and gene therapy of neurological disorders.
KW  - foamy virus
KW  - spumavirus
KW  - viral vector
KW  - gene therapy
KW  - vector tropism
KW  - bioimaging
KW  - hippocampus
Y1  - 2018
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-223379
VL  - 12
ER  - 
TY  - JOUR
A1  - Lindemann, Dirk
A1  - Rethwilm, Axel
T1  - Foamy Virus Biology and Its Application for Vector Development
JF  - Viruses
N2  - Spuma- or foamy viruses (FV), endemic in most non-human primates, cats, cattle and horses, comprise a special type of retrovirus that has developed a replication strategy combining features of both retroviruses and hepadnaviruses. Unique features of FVs include an apparent apathogenicity in natural hosts as well as zoonotically infected humans, a reverse transcription of the packaged viral RNA genome late during viral replication resulting in an infectious DNA genome in released FV particles and a special particle release strategy depending capsid and glycoprotein coexpression and specific interaction between both components. In addition, particular features with respect to the integration profile into the host genomic DNA discriminate FV from orthoretroviruses. It appears that some inherent properties of FV vectors set them favorably apart from orthoretroviral vectors and ask for additional basic research on the viruses as well as on the application in Gene Therapy. This review will summarize the current knowledge of FV biology and the development as a gene transfer system.
KW  - terminal gag domain
KW  - env leader protein
KW  - enhance viral transcription
KW  - subviral particle release
KW  - cell-cycle dependence
KW  - foamyviruses
KW  - retroviral vectors
KW  - LAD
KW  - Fanconi Anemia
KW  - cis-acting sequences
KW  - dna-binding protein
KW  - pol messenger-rna
KW  - reverse-transcriptase
KW  - gene-expression
Y1  - 2011
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-139811
VL  - 3
IS  - 5
ER  - 
TY  - JOUR
A1  - Berkhout, Ben
A1  - Bodem, Jochen
A1  - Erlwein, Otto
A1  - Herchenröder, Ottmar
A1  - Khan, Arifa S.
A1  - Lever, Andrew M. L.
A1  - Lindemann, Dirk
A1  - Linial, Maxine L.
A1  - Löchelt, Martin
A1  - McClure, Myra O.
A1  - Scheller, Carsten
A1  - Weiss, Robin A.
T1  - Obituary: Axel Rethwilm (1959–2014)
JF  - Retrovirology
N2  - No abstract available
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-120781
VL  - 11
IS  - 85
ER  -