TY  - JOUR
A1  - Langenhorst, Daniela
A1  - Haack, Stephanie
A1  - Göb, Selina
A1  - Uri, Anna
A1  - Lühder, Fred
A1  - Vanhove, Bernhard
A1  - Hünig, Thomas
A1  - Beyersdorf, Niklas
T1  - CD28 costimulation of T helper 1 cells enhances cytokine release in vivo
JF  - Frontiers in Immunology
N2  - Compared to naive T cells, differentiated T cells are thought to be less dependent on CD28 costimulation for full activation. To revisit the role of CD28 costimulation in mouse T cell recall responses, we adoptively transferred in vitro generated OT-II T helper (Th) 1 cells into C57BL/6 mice (Thy1.2\(^{+}\)) and then either blocked CD28–ligand interactions with Fab fragments of the anti-CD28 monoclonal antibody (mAb) E18 or deleted CD28 expression using inducible CD28 knock-out OT-II mice as T cell donors. After injection of ovalbumin protein in adjuvant into the recipient mice we observed that systemic interferon (IFN)γ release strongly depended on CD28 costimulation of the Th1 cells, while secondary clonal expansion was not reduced in the absence of CD28 costimulation. For human memory CD4\(^{+}\) T cell responses we also noted that cytokine release was reduced upon inhibition of CD28 costimulation. Together, our data highlight the so far underestimated role of CD28 costimulation for the reactivation of fully differentiated CD4\(^{+}\) T cells.
KW  - CD4\(^{+}\) T helper cells
KW  - T helper 1 cells
KW  - antigenic recall
KW  - CD28 costimulation
KW  - cytokine secretion
KW  - mouse
KW  - human
Y1  - 2018
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-176726
VL  - 9
IS  - 1060
ER  -