TY  - JOUR
A1  - Amatobi, Kelechi M.
A1  - Ozbek-Unal, Ayten Gizem
A1  - Schäbler, Stefan
A1  - Deppisch, Peter
A1  - Helfrich-Förster, Charlotte
A1  - Mueller, Martin J.
A1  - Wegener, Christian
A1  - Fekete, Agnes
T1  - The circadian clock is required for rhythmic lipid transport in Drosophila in interaction with diet and photic condition
JF  - Journal of Lipid Research
N2  - Modern lifestyle is often at odds with endogenously driven rhythmicity, which can lead to circadian disruption and metabolic syndrome. One signature for circadian disruption is a reduced or altered metabolite cycling in the circulating tissue reflecting the current metabolic status. Drosophila is a well-established model in chronobiology, but day-time dependent variations of transport metabolites in the fly circulation are poorly characterized. Here, we sampled fly hemolymph throughout the day and analyzed diacylglycerols (DGs), phosphoethanolamines (PEs) and phosphocholines (PCs) using LC-MS. In wild-type flies kept on sugar-only medium under a light-dark cycle, all transport lipid species showed a synchronized bimodal oscillation pattern with maxima at the beginning and end of the light phase which were impaired in period01 clock mutants. In wild-type flies under constant dark conditions, the oscillation became monophasic with a maximum in the middle of the subjective day. In strong support of clock-driven oscillations, levels of the targeted lipids peaked once in the middle of the light phase under time-restricted feeding independent of the time of food intake. When wild-type flies were reared on full standard medium, the rhythmic alterations of hemolymph lipid levels were greatly attenuated. Our data suggest that the circadian clock aligns daily oscillations of DGs, PEs, and PCs in the hemolymph to the anabolic siesta phase, with a strong influence of light on phase and modality.
KW  - hemolymph lipids
KW  - lipidomics
KW  - circadian rhythm
KW  - feeding
KW  - locomotor activity
KW  - light-driven metabolism
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-349961
VL  - 64
IS  - 10
ER  -