TY  - JOUR
A1  - Beyersdorf, Niklas
A1  - Werner, Sandra
A1  - Wolf, Nelli
A1  - Herrmann, Thomas
A1  - Kerkau, Thomas
T1  - Characterization of a New Mouse Model for Peripheral T Cell Lymphoma in Humans
JF  - PLoS One
N2  - Peripheral T cell lymphomas (PTCLs) are associated with a poor prognosis due to often advanced disease at the time of diagnosis and due to a lack of efficient therapeutic options. Therefore, appropriate animal models of PTCL are vital to improve clinical management of this disease. Here, we describe a monoclonal CD8\(^+\) CD4\(^−\) αβ T cell receptor Vβ2\(^+\) CD28\(^+\) T cell lymphoma line, termed T8-28. T8-28 cells were isolated from an un-manipulated adult BALB/c mouse housed under standard pathogen-free conditions. T8-28 cells induced terminal malignancy upon adoptive transfer into syngeneic BALB/c mice. Despite intracellular expression of the cytotoxic T cell differentiation marker granzyme B, T8-28 cells appeared to be defective with respect to cytotoxic activity as read-out in vitro. Among the protocols tested, only addition of interleukin 2 in vitro could partially compensate for the in vivo micro-milieu in promoting growth of the T8-28 lymphoma cells.
KW  - T cells
KW  - cytotoxic T cells
KW  - mouse models
KW  - interleukins
KW  - cell staining
KW  - lymphomas
KW  - fluorescence-activated cell sorting
KW  - lymph nodes
Y1  - 2011
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-137946
VL  - 6
IS  - 12
ER  - 
TY  - JOUR
A1  - Paletta, Daniel
A1  - Fichtner, Alina Suzann
A1  - Starick, Lisa
A1  - Porcelli, Steven A.
A1  - Savage, Paul B.
A1  - Herrmann, Thomas
T1  - Species Specific Differences of CD1d Oligomer Loading In Vitro
JF  - PLoS One
N2  - CD1d molecules are MHC class I-like molecules that present glycolipids to iNKT cells. The highly conserved interaction between CD1d:α-Galactosylceramide (αGC) complexes and the iNKT TCR not only defines this population of αβ T cells but can also be used for its direct identification. Therefore, CD1d oligomers are a widely used tool for iNKT cell related investigations. To this end, the lipid chains of the antigen have to be inserted into the hydrophobic pockets of the CD1d binding cleft, often with help of surfactants. In this study, we investigated the influence of different surfactants (Triton X-100, Tween 20, Tyloxapol) on in vitro loading of CD1d molecules derived from four different species (human, mouse, rat and cotton rat) with αGC and derivatives carrying modifications of the acyl-chain (DB01-1, PBS44) and a 6-acetamido-6-deoxy-addition at the galactosyl head group (PBS57). We also compared rat CD1d dimers with tetramers and staining of an iNKT TCR transductant was used as readout for loading efficacy. The results underlined the importance of CD1d loading efficacy for proper analysis of iNKT TCR binding and demonstrated the necessity to adjust loading conditions for each oligomer/glycolipid combination. The efficient usage of surfactants as a tool for CD1d loading was revealed to be species-specific and depending on the origin of the CD1d producing cells. Additional variation of surfactant-dependent loading efficacy between tested glycolipids was influenced by the acyl-chain length and the modification of the galactosyl head group with PBS57 showing the least dependence on surfactants and the lowest degree of species-dependent differences.
KW  - cell staining
KW  - major histocompatibility complex
KW  - binding analysis
KW  - oligomers
KW  - glycolipids
KW  - lipids
KW  - surfactants
KW  - T cells
Y1  - 2015
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-124879
VL  - 10
IS  - 11
ER  -