TY  - JOUR
A1  - Hong, Yunhan
A1  - Schartl, Manfred
T1  - Structure of the rainbow trout metallothionein A gene
N2  - To investigate the regulation of metallothionein-encoding genes in fish, we have isolated and sequenced the rainbow trout metallothionein-A-encoding gene (tMT-A) by polymerase chain reaction. This gene spans about 1.1 kb, consists of three exons and two introns, and has an A+ T-rieb 5' -region which contains a TATAAA signal, and two metal responsive elements (MREs). The transcription start point is centered around an A residue 81 nt upstream of the ATG codon.
KW  - Physiologische Chemie
KW  - Recombinant DNA ; polymerase chain reaction ; metallothionein gene ; rainbow trout ; fish
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-61679
ER  - 
TY  - JOUR
A1  - Winkler, Christoph
A1  - Hong, Yunhan
A1  - Wittbrodt, Joachim
A1  - Schartl, Manfred
T1  - Analysis of heterologous and homologous promoters and enhancers in vitro and in vivo by gene transfer into Japanese medaka (Oryzias latipes) and Xiphophorus
N2  - Efficient expression systems are required for analysis of gene regulation and function in teleost fish. To develop such systems, a nurober of inducible or constitutive promoter and enhancer sequences of fish or higher vertebrate origin were tested for activity in a variety of fish celllines andin embryos of the Japanese medaka fish (Oryzias latipes) and Xiphophorus. The activity of the different promoterenhancer combinations were quantitated. Considerable differences were found for some constructs if tested in vitro or in vivo. From the data obtained, a set of expression vectors for basic research as weH as for aquaculture purposes were established.
KW  - Schwertkärpfling
KW  - Japankärpfling
KW  - Gentransfer
KW  - Enhancer
KW  - Promotor
KW  - In vitro
KW  - In vivo
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-86796
ER  -