TY  - JOUR
A1  - Beyersdorf, Niklas
A1  - Werner, Sandra
A1  - Wolf, Nelli
A1  - Herrmann, Thomas
A1  - Kerkau, Thomas
T1  - Characterization of a New Mouse Model for Peripheral T Cell Lymphoma in Humans
JF  - PLoS One
N2  - Peripheral T cell lymphomas (PTCLs) are associated with a poor prognosis due to often advanced disease at the time of diagnosis and due to a lack of efficient therapeutic options. Therefore, appropriate animal models of PTCL are vital to improve clinical management of this disease. Here, we describe a monoclonal CD8\(^+\) CD4\(^−\) αβ T cell receptor Vβ2\(^+\) CD28\(^+\) T cell lymphoma line, termed T8-28. T8-28 cells were isolated from an un-manipulated adult BALB/c mouse housed under standard pathogen-free conditions. T8-28 cells induced terminal malignancy upon adoptive transfer into syngeneic BALB/c mice. Despite intracellular expression of the cytotoxic T cell differentiation marker granzyme B, T8-28 cells appeared to be defective with respect to cytotoxic activity as read-out in vitro. Among the protocols tested, only addition of interleukin 2 in vitro could partially compensate for the in vivo micro-milieu in promoting growth of the T8-28 lymphoma cells.
KW  - T cells
KW  - cytotoxic T cells
KW  - mouse models
KW  - interleukins
KW  - cell staining
KW  - lymphomas
KW  - fluorescence-activated cell sorting
KW  - lymph nodes
Y1  - 2011
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-137946
VL  - 6
IS  - 12
ER  -