TY  - JOUR
A1  - Wolfahrt, Sonja
A1  - Herman, Sandra
A1  - Scholz, Claus-Jürgen
A1  - Sauer, Georg
A1  - Deissler, Helmut
T1  - Identification of alternative transcripts of rat CD9 expressed by tumorigenic neural cell lines and in normal tissues
JF  - Genetics and Molecular Biology
N2  - CD9 is the best-studied member of the tetraspanin family of transmembrane proteins. It is involved in various fundamental cellular processes and its altered expression is a characteristic of malignant cells of different origins. Despite numerous investigations confirming its fundamental role, the heterogeneity of CD9 or other tetraspanin proteins was considered only to be caused by posttranslational modification, rather than alternative splicing. Here we describe the first identification of CD9 transcript variants expressed by cell lines derived from fetal rat brain cells. Variant mRNA-B lacks a potential translation initiation codon in the alternative exon 1 and seems to be characteristic of the tumorigenic BT cell lines. In contrast, variant mRNA-C can be translated from a functional initiation codon located in its extended exon 2, and substantial amounts of this form detected in various tissues suggest a contribution to CD9 functions. From the alternative sequence of variant C, a different membrane topology ( 5 transmembrane domains) and a deviating spectrum of functions can be expected.
KW  - tetraspanin
KW  - CD9
KW  - antigen
KW  - cancer
KW  - noncoding RNAs
KW  - nervous system
KW  - poor prognosis
KW  - tetraspanin protein
KW  - transcript
KW  - splice variant
KW  - membrane topology
Y1  - 2013
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-131801
VL  - 36
IS  - 2
ER  - 
TY  - JOUR
A1  - Dimastrogiovanni, Daniela
A1  - Fröhlich, Kathrin S.
A1  - Bandyra, Katarzyna J.
A1  - Bruce, Heather A.
A1  - Hohensee, Susann
A1  - Vogel, Jörg
A1  - Luisi, Ben F.
T1  - Recognition of the small regulatory RNA RydC by the bacterial Hfq protein
JF  - eLife
N2  - Bacterial small RNAs (sRNAs) are key elements of regulatory networks that modulate gene expression. The sRNA RydC of Salmonella sp. and Escherichia coli is an example of this class of riboregulators. Like many other sRNAs, RydC bears a 'seed' region that recognises specific transcripts through base-pairing, and its activities are facilitated by the RNA chaperone Hfq. The crystal structure of RydC in complex with E. coli Hfq at 3.48 angstrom resolution illuminates how the protein interacts with and presents the sRNA for target recognition. Consolidating the protein-RNA complex is a host of distributed interactions mediated by the natively unstructured termini of Hfq. Based on the structure and other data, we propose a model for a dynamic effector complex comprising Hfq, small RNA, and the cognate mRNA target.
KW  - Hfq
KW  - small RNA
KW  - natively unstructured protein
KW  - protein-RNA recognition
KW  - gene regulation
KW  - Escherichia coli-Hfq
KW  - SM-like protein
KW  - messenger-RNA
KW  - chaperone Hfq
KW  - target recognition
KW  - noncoding RNAs
KW  - interaction surfaces
KW  - crystal-structures
KW  - soluble-RNAs
KW  - C-Terminus
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-114191
SN  - 2050-084X
VL  - 3
IS  - e05375
ER  -