TY  - JOUR
A1  - Pereira, A. R.
A1  - Trivanović, D.
A1  - Herrmann, M.
T1  - Approaches to mimic the complexity of the skeletal mesenchymal stem/stromal cell niche in vitro
JF  - European Cells and Materials
N2  - Mesenchymal stem/stromal cells (MSCs) are an essential element of most modern tissue engineering and regenerative medicine approaches due to their multipotency and immunoregulatory functions. Despite the prospective value of MSCs for the clinics, the stem cells community is questioning their developmental origin, in vivo localization, identification, and regenerative potential after several years of far-reaching research in the field. Although several major progresses have been made in mimicking the complexity of the MSC niche in vitro, there is need for comprehensive studies of fundamental mechanisms triggered by microenvironmental cues before moving to regenerative medicine cell therapy applications. The present comprehensive review extensively discusses the microenvironmental cues that influence MSC phenotype and function in health and disease – including cellular, chemical and physical interactions. The most recent and relevant illustrative examples of novel bioengineering approaches to mimic biological, chemical, and mechanical microenvironmental signals present in the native MSC niche are summarized, with special emphasis on the forefront techniques to achieve bio-chemical complexity and dynamic cultures. In particular, the skeletal MSC niche and applications focusing on the bone regenerative potential of MSC are addressed. The aim of the review was to recognize the limitations of the current MSC niche in vitro models and to identify potential opportunities to fill the bridge between fundamental science and clinical application of MSCs.
KW  - Mesenchymal stem/stromal cells
KW  - skeletal progenitor cells
KW  - niche
KW  - in vitro models
KW  - bone
KW  - tissue engineering
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-268823
SN  - 1473-2262
VL  - 37
ER  - 
TY  - JOUR
A1  - Canesi, Margherita
A1  - Giordano, Rosaria
A1  - Lazzari, Lorenza
A1  - Isalberti, Maurizio
A1  - Isaias, Ioannis Ugo
A1  - Benti, Riccardo
A1  - Rampini, Paolo
A1  - Marotta, Giorgio
A1  - Colombo, Aurora
A1  - Cereda, Emanuele
A1  - Dipaola, Mariangela
A1  - Montemurro, Tiziana
A1  - Vigano, Mariele
A1  - Budelli, Silvia
A1  - Montelatici, Elisa
A1  - Lavazza, Cristiana
A1  - Cortelezzi, Agostino
A1  - Pezzoli, Gianni
T1  - Finding a new therapeutic approach for no-option Parkinsonisms: mesenchymal stromal cells for progressive supranuclear palsy
JF  - Journal of Translational Medicine
N2  - Background: The trophic, anti-apoptotic and regenerative effects of bone marrow mesenchymal stromal cells (MSC) may reduce neuronal cell loss in neurodegenerative disorders. 

Methods: We used MSC as a novel candidate therapeutic tool in a pilot phase-I study for patients affected by progressive supranuclear palsy (PSP), a rare, severe and no-option form of Parkinsonism. Five patients received the cells by infusion into the cerebral arteries. Effects were assessed using the best available motor function rating scales (UPDRS, Hoehn and Yahr, PSP rating scale), as well as neuropsychological assessments, gait analysis and brain imaging before and after cell administration. 

Results: One year after cell infusion, all treated patients were alive, except one, who died 9 months after the infusion for reasons not related to cell administration or to disease progression (accidental fall). In all treated patients motor function rating scales remained stable for at least six-months during the one-year follow-up. 

Conclusions: We have demonstrated for the first time that MSC administration is feasible in subjects with PSP. In these patients, in whom deterioration of motor function is invariably rapid, we recorded clinical stabilization for at least 6 months. These encouraging results pave the way to the next randomized, placebo-controlled phase-II study that will definitively provide information on the efficacy of this innovative approach.
KW  - Progressive supranuclear palsy
KW  - Mesenchymal stem/stromal cells
KW  - Cell therapy
KW  - Regenerative medicine
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-165725
VL  - 14
IS  - 127
ER  -