TY - THES A1 - Ulrich, Johannes T1 - Molekulare Charaktierisierung einer DyP-Typ Peroxidase des Humanparasiten \(Echinococcus\) \(multilocularis\) T1 - Molecular characterisation of a DyP-type peroxidase of the human parasite \(Echinococcus\) \(multilocularis\) N2 - Die Alveoläre Echinokokkose (AE) ist eine tödliche Infektionserkrankung, die durch den parasitären Plattwurm Echinococcus multilocularis verursacht wird. Genomanalysen von E. multilocularis ergaben ein Gen, das laut Vorhersage für eine DyP-Typ Peroxidase codiere. Ziel dieser Arbeit ist die biologische Funktion des codierten Enzyms besser zu verstehen und Hinweise auf eine mögliche Rolle in der Abwehr von Reaktiven Sauerstoffspezies (ROS) zu erlangen. Das Gen wurde heterolog in E. Coli exprimiert und molekulare Charakteristika des Gens mit bioinformatischen und molekularbiologischen Methoden untersucht. Quantitative RT-PCR Untersuchungen gaben Aufschluss über das Transkriptprofil von emipox in unterschiedlichen Entwicklungsstadien von E. mulitlocularis. Mittels Whole-Mount In Situ-Hybridisierung (WMISH) wurden die Transkripte zudem lokalisiert und ihre Beziehung zum Stammzellsystem von E. multilocularis näher untersucht. Die Zugehörigkeit von EmIPOX zur Gruppe der DyP-Typ Peroxidasen wurde bestätigt. Homologe beim Menschen kommen nicht vor. Es konnte nachgewiesen werden, dass Transkripte von emipox auch, aber keinesfalls ausschließlich, in Stammzellen vorliegen. Überdurchschnittlich viele Transkripte liegen im aktivierten Protoscolex und im Metacestoden ex vivo aus einer infizierten Wirtsleber vor. Untersuchungen zur Enzymaktivität von EmIPOX zeigten neben einer Peroxidase- auch eine Katalaseaktivität. Die vorliegende Arbeit ist die erste Charakterisierung einer DyP-Typ Peroxidase bei Tieren. Sie legt nahe, dass EmIPOX eine Rolle in der Entgiftung von ROS in E. multilocularis spielt und stellt den Charakter von EmIPOX als potenzieller pharmakologischer Zielstruktur heraus. N2 - Alveolar echinococcosis (AE) is a fatal infectious disease caused by the parasitic flatworm Echinococcus multilocularis. Genome analyses of E. multilocularis revealed a gene predicted to encode a DyP-type peroxidase. The aim of this work is to better understand the biological function of the encoded enzyme and to obtain information on a possible role in the defence against reactive oxygen species (ROS). The gene was heterologously expressed in E. Coli and molecular characteristics of the gene were investigated using bioinformatic and molecular biological methods. Quantitative RT-PCR analyses provided information on the transcript profile of emipox in different developmental stages of E. mulitlocularis. Whole-mount in situ hybridisation (WMISH) was also used to localise the transcripts and investigate their relationship to the stem cell system of E. multilocularis. The affiliation of EmIPOX to the group of DyP-type peroxidases was confirmed. There are no homologues in humans. It has been shown that transcripts of emipox are also, but by no means exclusively, present in stem cells. An above-average number of transcripts are present in the activated protoscolex and in the metacestode ex vivo from an infected host liver. Investigations into the enzyme activity of EmIPOX revealed both peroxidase and catalase activity. The present work is the first characterisation of a DyP-type peroxidase in animals. It suggests that EmIPOX plays a role in the detoxification of ROS in E. multilocularis and highlights the character of EmIPOX as a potential pharmacological target. KW - Fuchsbandwurm KW - Oxidativer Stress KW - Peroxidase KW - Parasitäre Krankheit KW - Alveoläre Echinokokkose KW - alveolar echinococcosis KW - oxidative stress KW - DyP-type peroxidase KW - DyP-Typ Peroxidase KW - cestode KW - Bandwurm KW - Plathelminthes KW - flat worms KW - neglected tropical disease KW - katalase KW - Bandwürmer KW - Plattwürmer Y1 - 2024 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-357143 ER - TY - JOUR A1 - Stijnis, Kees A1 - Dijkmans, Anneke C. A1 - Bart, Aldert A1 - Brosens, Lodewijk A. A. A1 - Muntau, Birgit A1 - Schoen, Christoph A1 - Barth, Thomas F. A1 - van Gulik, Thomas A1 - van Gool, Tom A1 - Grobusch, Martin P. A1 - Tappe, Dennis T1 - Echinococcus vogeli in Immigrant from Suriname to the Netherlands JF - Emerging Infectious Diseases KW - alveolar echinococcosis KW - monoclonal antibody KW - multilocularis Y1 - 2015 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-143953 VL - 21 IS - 3 ER - TY - JOUR A1 - Atanasov, Georgi A1 - Benkert, Christoph A1 - Thelen, Armin A1 - Tappe, Dennis A1 - Frosch, Matthias A1 - Teichmann, Dieter A1 - Barth, Thomas F. E. A1 - Wittekind, Christian A1 - Schubert, Stefan A1 - Jonas, Sven T1 - Alveolar echinococcosis-spreading disease challenging clinicians: A case report and literature review JF - World Journal of Gastroenterology N2 - Human alveolar echinococcosis (AE) is a potentially deadly disease; recent studies have shown that the endemic area of Echinococcus multilocularis, its causative agent, is larger than previously known. This disease has low prevalence and remains underreported in Europe. Emerging clinical data show that diagnostic difficulties are still common. We report on a 76-year old patient suffering from AE lesions restricted to the left lobe of the liver who underwent a curative extended left hemihepatectomy. Prior to the resection a liver biopsy under the suspicion of an atypical malignancy was performed. After the intervention he developed a pseudoaneurysm of the hepatic artery that was successfully coiled. Surprisingly, during surgery, the macroscopic appearance of the tumour revealed a growth pattern that was rather typical for cystic echinococcosis (CE), i.e., a gross tumour composed of multiple large vesicles with several centimeters in diameter. In addition, there were neither extensive adhesions nor infiltrations of the neighboring pancreas and diaphragm as was expected from previous imaging results. The unexpected diagnosis of AE was confirmed by definite histopathology, specific polymerase chain reaction and serology results. This is a rare case of unusual macroscopic presentation of AE that posed immense diagnostic challenges and had an eventful course. To our knowledge this is the first case of an autochthonous infection in this particular geographic area of Germany, the federal state of Saxony. This report may provide new hints for an expanding area of risk for AE and emphasizes the risk of complications in the scope of diagnostic procedures and the limitations of modern radiological imaging. KW - hemihepatectomy KW - multilocularis KW - Germany KW - Europe KW - liver KW - alveolar echinococcosis KW - echinococcus multilocularis KW - autochthonous infection KW - liver resection Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-131525 VL - 19 IS - 26 ER -