TY  - THES
A1  - Leipold-Büttner, Carsten
T1  - Immunhistochemische Darstellung von spannungsabhängigen Calciumkanälen an peripheren afferenten Nervenfasern im Kniegelenk der Maus
T1  - Demonstration of voltage dependent calcium channels on calcitonin gene- related peptide-immunoreactive nerve fibres in the mouse knee joint
N2  - The afferent function (excitability) and efferent function (release of neuropeptides) of primary afferent nerve fibres is based on Ca2+-influx. Aim of the present study was to examine the presence of L-and N-type Ca2+-channels at sensory nerve fibres in the mouse knee joint capsule. Specific fluorescent labelled channel blocker and antisera against these channel subtypes were combined with an immunhistochemical staining for calcitonin gene-related peptide (CGRP), a neuropeptide that is wildly distributed in primary afferents. There was nearly complete colocalisation of calcitonin gene-related peptide immunoreactivity and the binding of conotoxin GVIA or BODIPY- verapamil demonstrating the presence of N-type and L-type Ca2+-channels, respectively. These data were further confirmed by identical results obtained after an immunohistochemical demonstration of the two channel subtypes at the peptidergic nerve fibres.
KW  - CGRP
KW  - Nozizeption
KW  - Calciumkanäle
KW  - Afferenzen
KW  - CGRP
KW  - calcium channel
KW  - nociception
KW  - primary afferent
Y1  - 2005
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-17894
ER  -