TY  - THES
A1  - Xiao, Zheng
T1  - Blimp-1 Regulates Terminal Differentiation of T Cells
T1  - Regulation der terminalen Differenzierung von T-Zellen durch Blimp-1
N2  - The transcriptional repressor-Blimp-1 terminates differentiation of B lymphocytes as well as myeloid cells. Our data show that Blimp-1 is highly expressed in freshly isolated murine primary T lymphocytes, particularly its minor splice variant. Ectopic expression of Blimp-1 by retroviral transduction neither dramatically altered secretion of IFN-ã or IL-4 nor did it induce the ability to suppress as regulatory T cells. However, induction of Blimp-1 resulted in not only a significant reduction in the production of IL-2 but also an inability to proliferate as well as in the reduced viability. These results demonstrate that Blimp-1 might mark end stages of lineage differentiation in T cells.
KW  - Blimp-1
KW  - T- Zelle
KW  - Regluation
KW  - Blimp-1
KW  - T cell
KW  - Regulation
Y1  - 2004
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-10530
ER  - 
TY  - THES
A1  - Schneider, Evelin
T1  - Einfluß von Blimp-1 auf die Interferonproduktion und Virusvermehrung
T1  - Influence of Blimp-1 on interferon production and virus replication
N2  - Diese Dissertation befaßt sich mit der Frage, ob der Transkriptionsfaktor Blimp-1 ebenso wie sein humanes Äquivalent PRDI-BF1 die Interferonproduktion reprimiert und somit die Virusvermehrung in Zellen erleichtert. An Blimp-1 exprimierenden L929-Zellen wurde die Interferonproduktion mit Hilfe des RNase Protection Assay auf mRNA-Ebene quantifiziert und die Virusvermehrung im Plaquetest untersucht. In beiden Fällen bestand kein signifikanter Unterschied zu einer Kontrollpopulation ohne Blimp-1-Expression, so daß die funktionelle Äquivalenz der beiden Transkriptionsfaktoren angezweifelt werden kann.
N2  - This thesis treats with the question whether the transcription factor Blimp-1 inhibits interferon expression and facilitates intracellular virus replication, as has been shown for its human equivalent PRDI-BF1. In L929 cells the RNase Protection Assay was used to quantify the transcription of interferon mRNA, and virus replication was shown in the plaque-test. In both cases there was no significant difference between cells with and without Blimp-1 expression, which indicates that the functional equivalence of Blimp-1 and PRDI-BF1 may be questioned.
KW  - Blimp-1
KW  - Interferone
KW  - Blimp-1
KW  - interferon
Y1  - 2004
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-9010
ER  -