TY  - JOUR
A1  - Kurz, Felix T.
A1  - Kampf, Thomas
A1  - Buschle, Lukas R.
A1  - Schlemmer, Heinz-Peter
A1  - Heiland, Sabine
A1  - Bendszus, Martin
A1  - Ziener, Christian H.
T1  - Microstructural Analysis of Peripheral Lung Tissue through CPMG Inter-Echo Time R2 Dispersion
JF  - PLoS One
N2  - Since changes in lung microstructure are important indicators for (early stage) lung pathology, there is a need for quantifiable information of diagnostically challenging cases in a clinical setting, e.g. to evaluate early emphysematous changes in peripheral lung tissue. Considering alveoli as spherical air-spaces surrounded by a thin film of lung tissue allows deriving an expression for Carr-Purcell-Meiboom-Gill transverse relaxation rates R-2 with a dependence on inter-echo time, local air-tissue volume fraction, diffusion coefficient and alveolar diameter, within a weak field approximation. The model relaxation rate exhibits the same hyperbolic tangent dependency as seen in the Luz-Meiboom model and limiting cases agree with Brooks et al. and Jensen et al. In addition, the model is tested against experimental data for passively deflated rat lungs: the resulting mean alveolar radius of RA = 31.46 \(\pm\) 13.15 \(\mu\)m is very close to the literature value (similar to 34 \(\mu\)m). Also, modeled radii obtained from relaxometer measurements of ageing hydrogel foam (that mimics peripheral lung tissue) are in good agreement with those obtained from mu CT images of the same foam (mean relative error: 0.06 \(\pm\) 0.01). The model's ability to determine the alveolar radius and/or air volume fraction will be useful in quantifying peripheral lung microstructure.
KW  - transverse relaxation
KW  - number
KW  - HE-3 diffusion MRI
KW  - magnetic-resonance behavior
KW  - hyperpolarized HE-3
KW  - Bessle functions
KW  - self-diffusion
KW  - alveolar
KW  - field
KW  - morphometry
Y1  - 2015
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-138345
VL  - 10
IS  - 11
ER  - 
TY  - JOUR
A1  - Munz, Eberhard
A1  - Jakob, Peter M.
A1  - Borisjuk, Ljudmilla
T1  - The potential of nuclear magnetic resonance to track lipids in planta
JF  - Biochimie
N2  - Nuclear Magnetic Resonance (NMR) provides a highly flexible platform for non invasive analysis and imaging biological samples, since the manipulation of nuclear spin allows the tailoring of experiments to maximize the informativeness of the data. MRI is capable of visualizing a holistic picture of the lipid storage in living plant/seed. This review has sought to explain how the technology can be used to acquire functional and physiological data from plant samples, and how to exploit it to characterize lipid deposition in vivo. At the same time, we have referred to the current limitations of NMR technology as applied to plants, and in particular of the difficulty of transferring methodologies optimized for animal/medical subjects to plant ones. A forward look into likely developments in the field is included, anticipating its key future role in the study of living plant.
KW  - coconut cocos-nucifera
KW  - H-1-NMR spectroscopy
KW  - NMR-spectroscopy
KW  - camelina-sativa
KW  - high-throughput
KW  - oil storage
KW  - seeds
KW  - accumulation
KW  - field
KW  - metabolism
KW  - NMR
KW  - Lipid
KW  - MRI
KW  - CSI
KW  - Plants
KW  - Seeds
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-186828
VL  - 130
ER  -