TY  - JOUR
A1  - Eder, Sascha
A1  - Hollmann, Claudia
A1  - Mandasari, Putri
A1  - Wittmann, Pia
A1  - Schumacher, Fabian
A1  - Kleuser, Burkhard
A1  - Fink, Julian
A1  - Seibel, Jürgen
A1  - Schneider-Schaulies, Jürgen
A1  - Stigloher, Christian
A1  - Beyersdorf, Niklas
A1  - Dembski, Sofia
T1  - Synthesis and characterization of ceramide-containing liposomes as membrane models for different T cell subpopulations
JF  - Journal of Functional Biomaterials
N2  - A fine balance of regulatory (T\(_{reg}\)) and conventional CD4\(^+\) T cells (T\(_{conv}\)) is required to prevent harmful immune responses, while at the same time ensuring the development of protective immunity against pathogens. As for many cellular processes, sphingolipid metabolism also crucially modulates the T\(_{reg}\)/T\(_{conv}\) balance. However, our understanding of how sphingolipid metabolism is involved in T cell biology is still evolving and a better characterization of the tools at hand is required to advance the field. Therefore, we established a reductionist liposomal membrane model system to imitate the plasma membrane of mouse T\(_{reg}\) and T\(_{conv}\) with regards to their ceramide content. We found that the capacity of membranes to incorporate externally added azide-functionalized ceramide positively correlated with the ceramide content of the liposomes. Moreover, we studied the impact of the different liposomal preparations on primary mouse splenocytes in vitro. The addition of liposomes to resting, but not activated, splenocytes maintained viability with liposomes containing high amounts of C\(_{16}\)-ceramide being most efficient. Our data thus suggest that differences in ceramide post-incorporation into T\(_{reg}\) and T\(_{conv}\) reflect differences in the ceramide content of cellular membranes.
KW  - liposome
KW  - ceramide
KW  - cell membrane model
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-286130
SN  - 2079-4983
VL  - 13
IS  - 3
ER  - 
TY  - JOUR
A1  - Avota, Elita
A1  - Bodem, Jochen
A1  - Chithelen, Janice
A1  - Mandasari, Putri
A1  - Beyersdorf, Niklas
A1  - Schneider-Schaulies, Jürgen
T1  - The Manifold Roles of Sphingolipids in Viral Infections
JF  - Frontiers in Physiology
N2  - Sphingolipids are essential components of eukaryotic cells. In this review, we want to exemplarily illustrate what is known about the interactions of sphingolipids with various viruses at different steps of their replication cycles. This includes structural interactions during entry at the plasma membrane or endosomal membranes, early interactions leading to sphingolipid-mediated signal transduction, interactions with internal membranes and lipids during replication, and interactions during virus assembly and budding. Targeted interventions in sphingolipid metabolism – as far as they can be tolerated by cells and organisms – may open novel possibilities to support antiviral therapies. Human immunodeficiency virus type 1 (HIV-1) infections have intensively been studied, but for other viral infections, such as influenza A virus (IAV), measles virus (MV), hepatitis C virus (HCV), dengue virus, Ebola virus, and severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), investigations are still in their beginnings. As many inhibitors of sphingolipid metabolism are already in clinical use against other diseases, repurposing studies for applications in some viral infections appear to be a promising approach.
KW  - sphingolipid
KW  - ceramide
KW  - sphingosine-1-phosphate
KW  - plasma membrane
KW  - virus entry
KW  - virus replication
KW  - virus budding
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-246975
SN  - 1664-042X
VL  - 12
ER  -