TY  - JOUR
A1  - Sebald, Walter
A1  - Bücher, T.
A1  - Olbrich, B.
A1  - Kaudewitz, F.
T1  - Electrophoretic pattern of and amino acid incorporation in vitro into the insoluble mitochondrial protein of neurospora crassa wild type and mi-1 mutant
N2  - No abstract available
KW  - Biochemie
Y1  - 1968
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-62926
ER  - 
TY  - JOUR
A1  - Sebald, Walter
A1  - Hofstötter, T.
A1  - Hacker, D.
A1  - Bücher, T.
T1  - Incorporation of amino acids into mitochondrial protein of the flight muscle of Locusta migratoria in vitro and in vivo in the presence of cycloheximide
N2  - No abstract available
KW  - Biochemie
Y1  - 1969
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-62919
ER  - 
TY  - JOUR
A1  - Sebald, Walter
A1  - Schwab, A. J.
A1  - Bücher, T.
T1  - Cycloheximide resistant amino acid incorporation into mitochondrial protein from Neurospora crassa in vivo
N2  - No abstract available
KW  - Biochemie
Y1  - 1969
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-62900
ER  - 
TY  - JOUR
A1  - Neupert, W.
A1  - Sebald, Walter
A1  - Schwab, A. J.
A1  - Pfaller, A.
A1  - Bücher, T.
T1  - Puromycin sensitivity of ribosomal label after incorporation of \(^{14}\)C-labelled amino acids into isolated mitochondria from Neurospora crassa
N2  - Radioactive amino acids were incorporated into isolated mitochondria from Neurospora crassa. Then the mitochondrial ribosomes were isolated and submitted to density gradient centrifugation. A preferential labelling of polysomes was observed. However, when the mitochondrial suspension was treated with puromycin after amino acid incorporation, no radioactivity could be detected in either the monosomes or the polysomes. The conclusion is drawn that isolated mitochondria under these conditions do not incorporate significant amounts of amino acids into proteins of their ribosomes.
KW  - Biochemie
Y1  - 1969
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-62899
ER  - 
TY  - JOUR
A1  - Neupert, W.
A1  - Sebald, Walter
A1  - Schwab, A. J.
A1  - Massinger, P.
A1  - Bücher, T.
T1  - Incorporation in vivo of \(^{14}\)C-labelled amino acids into the proteins of mitochondrial ribosomes from Neurospora crassa sensitive to cycloheximide and insensitive to Chloramphenicol
N2  - Radioactive amino acids were incorporated in vivo into N eurospora crassa cells, and the mitochondrial ribosomes were isolated. The incorporation of radioactivity into the proteins of these ribosomes was inhibited by cycloheximide, but not by chloramphenicol. It is therefore concluded that these proteins are synthesized on the cycloheximide sensitive and chloramphenicol insensitive cytoplasmic ribosomes.
KW  - Biochemie
Y1  - 1969
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-62884
ER  - 
TY  - JOUR
A1  - Weiss, H.
A1  - Sebald, Walter
A1  - Bücher, T.
T1  - Cycloheximide resistant incorporation of amino acids into a polypeptide of the cytochrome oxidase of Neurospora crassa
N2  - Radioaetive leueine was ineorporated by N eurospora crassa mitoehondria in vivo in the presence of cyeloheximide. When the membrane protein of these mitochondria was ehromatographieally separated on oleyl polymethaerylie aeid resin, & nurober of fraetions were obtained whieh differ with respeet to their eontents of radioaetivity and eytoehromes. The highest speeifie radioaetivity was found in the fraction eontaining eytoehrome aa3• This fraetion proved to be a pure and enzymatically aetive cytoehrome oxidase. Its ratio of absorbanee at 280 nm (ox)/ 443 nm (red.) was 2.1. By means of sodium dodeeylsulfate gel-electrophoresis, this enzymewas separated into five polypeptides with molecular weights of 30000, 20000, 13000, 10000, and 8000. Only the polypeptide with the molecular weight 20000 displayed a high specific radioaetivity.
KW  - Biochemie
Y1  - 1971
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-62866
ER  -