TY  - JOUR
A1  - Von Gaudecker, Brita
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, Wolfgang
T1  - Immunoelectron microscopic localization of MHC structures in isolated pancreatic rat islets
N2  - An immunogold-silver enhancement technique, which combines effective labeling of viable isolated islets with the ultrastructural resolution of cytological details, was applied in electron microscopy to identify major histocompatibility complex (MHC) structures on islet cells. Incubation of freshly isolated islets from CAP (RT1C) and LEW (RT1') rats with OX18, an MHC class I antibody, showed strong positive reactivity in macrophages and/or dendritic-like cells (M0-DCs) and vascular endothelial cells (VEs) and a comparatively weaker reactivity in endocrine a-, p-, and 8-ce"s. With MHC class" antibody OX6 (anti-I-A), M0-DCs were strongly labeled in both rat strains on the surface and on internal structures. Three of five particularly high titered batches of OX6 revealed MHC class" expression on VE and p-ce"s. Four days of in vitro culture in combination with a high concentration of glucose and interferon-'Y induced strong enhancement of MHC class I structures and, to a lesser extent, class " structures on p-ce"s.
KW  - Immunbiologie
Y1  - 1989
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45596
ER  - 
TY  - GEN
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Identification and Manipulation of Human Islet Alloimmunogenicity: Morphologic and Functional in Vitro Studies with Various Islet Preparations
N2  - No abstract available
KW  - Immunbiologie
Y1  - 1989
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45584
ER  - 
TY  - JOUR
A1  - Winoto-Morbach, Supandi
A1  - Ulrichs, Karin
A1  - Leyhausen, Gaby
A1  - Müller-Ruchholtz, Wolfgang
T1  - New principle for large-scale preparation of purified human pancreas islets
N2  - Because successful human islet transplantation requires large quantities of viable islets that must be separated from the highly immunogenic exocrine tissue and because handpicking is too time-consuming and laborious to be clinically relevant, a new approach for solving this problem has been established in rat models. It is based on the principle that magnetic microspheres (MMSs) coupled to lectins with binding specificity for the exocrine tissue portion are trapped in an electromagnetic field, thus providing effluent islets of a high degree of purity. In this study our aim was to adapt this princip'le to human islet preparations. In this context our prime interest was focused on a lectin suitable for human pancreatic tissue. Of 19 different lectins tested, only 1, Wisteria floribunda agglutinin (WFA), is suitable, as shown by immunofluorescence, MMS-Iectin binding, and magnetic separation
KW  - Immunbiologie
Y1  - 1989
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45600
ER  -