TY  - JOUR
A1  - El-Keredy, Amira
A1  - Schleyer, Michael
A1  - König, Christian
A1  - Ekim, Aslihan
A1  - Gerber, Bertram
T1  - Behavioural Analyses of Quinine Processing in Choice, Feeding and Learning of Larval Drosophila
JF  - PLoS One
N2  - Gustatory stimuli can support both immediate reflexive behaviour, such as choice and feeding, and can drive internal reinforcement in associative learning. For larval Drosophila, we here provide a first systematic behavioural analysis of these functions with respect to quinine as a study case of a substance which humans report as "tasting bitter". We describe the dose-effect functions for these different kinds of behaviour and find that a half-maximal effect of quinine to suppress feeding needs substantially higher quinine concentrations (2.0 mM) than is the case for internal reinforcement (0.6 mM). Interestingly, in previous studies (Niewalda et al. 2008, Schipanski et al 2008) we had found the reverse for sodium chloride and fructose/sucrose, such that dose-effect functions for those tastants were shifted towards lower concentrations for feeding as compared to reinforcement, arguing that the differences in dose-effect function between these behaviours do not reflect artefacts of the types of assay used. The current results regarding quinine thus provide a starting point to investigate how the gustatory system is organized on the cellular and/or molecular level to result in different behavioural tuning curves towards a bitter tastant.
KW  - honeybees
KW  - chemosensory system
KW  - bitter taste
KW  - melanogaster
KW  - receptor
KW  - reward
KW  - brain
KW  - organization
KW  - architecture
KW  - perception
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-130811
VL  - 7
IS  - 7
ER  - 
TY  - JOUR
A1  - Nanguneri, Siddharth
A1  - Flottmann, Benjamin
A1  - Horstmann, Heinz
A1  - Heilemann, Mike
A1  - Kuner, Thomas
T1  - Three-Dimensional, Tomographic Super-Resolution Fluorescence Imaging of Serially Sectioned Thick Samples
JF  - PLoS One
N2  - Three-dimensional fluorescence imaging of thick tissue samples with near-molecular resolution remains a fundamental challenge in the life sciences. To tackle this, we developed tomoSTORM, an approach combining single-molecule localization-based super-resolution microscopy with array tomography of structurally intact brain tissue. Consecutive sections organized in a ribbon were serially imaged with a lateral resolution of 28 nm and an axial resolution of 40 nm in tissue volumes of up to 50 \(\mu\)mx50\(\mu\)mx2.5\(\mu\)m. Using targeted expression of membrane bound (m)GFP and immunohistochemistry at the calyx of Held, a model synapse for central glutamatergic neurotransmission, we delineated the course of the membrane and fine-structure of mitochondria. This method allows multiplexed super-resolution imaging in large tissue volumes with a resolution three orders of magnitude better than confocal microscopy.
KW  - architecture
KW  - rat calyx
KW  - in-vivo
KW  - microscopy
KW  - resolution
KW  - proteins
KW  - transmission
KW  - ultrastructure
KW  - reconstruction
KW  - localization
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-134434
VL  - 7
IS  - 5
ER  -