TY  - JOUR
A1  - Kunz, Tobias C.
A1  - Götz, Ralph
A1  - Sauer, Markus
A1  - Rudel, Thomas
T1  - Detection of chlamydia developmental forms and secreted effectors by expansion microscopy
JF  - Frontiers in Cellular and Infection Microbiology
N2  - Expansion microscopy (ExM) is a novel tool to improve the resolution of fluorescence-based microscopy that has not yet been used to visualize intracellular pathogens. Here we show the expansion of the intracellular pathogen Chlamydia trachomatis, enabling to differentiate its two distinct forms, catabolic active reticulate bodies (RB) and infectious elementary bodies (EB), on a conventional confocal microscope. We show that ExM enables the possibility to precisely locate chlamydial effector proteins, such as CPAF or Cdu1, within and outside of the chlamydial inclusion. Thus, we claim that ExM offers the possibility to address a broad range of questions and may be useful for further research on various intracellular pathogens.
KW  - expansion microscopy
KW  - chlamydia
KW  - secreted effectors
KW  - developmental forms
KW  - superresolution
KW  - imaging
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-195716
SN  - 2235-2988
VL  - 9
IS  - 276
ER  -