TY  - JOUR
A1  - Sporbert, Anje
A1  - Cseresnyes, Zoltan
A1  - Heidbreder, Meike
A1  - Domaing, Petra
A1  - Hauser, Stefan
A1  - Kaltschmidt, Barbara
A1  - Kaltschmidt, Christian
A1  - Heilemann, Mike
A1  - Widera, Darius
T1  - Simple Method for Sub-Diffraction Resolution Imaging of Cellular Structures on Standard Confocal Microscopes by Three-Photon Absorption of Quantum Dots
JF  - PLoS ONE
N2  - This study describes a simple technique that improves a recently developed 3D sub-diffraction imaging method based on three-photon absorption of commercially available quantum dots. The method combines imaging of biological samples via tri-exciton generation in quantum dots with deconvolution and spectral multiplexing, resulting in a novel approach for multi-color imaging of even thick biological samples at a 1.4 to 1.9-fold better spatial resolution. This approach is realized on a conventional confocal microscope equipped with standard continuous-wave lasers. We demonstrate the potential of multi-color tri-exciton imaging of quantum dots combined with deconvolution on viral vesicles in lentivirally transduced cells as well as intermediate filaments in three-dimensional clusters of mouse-derived neural stem cells (neurospheres) and dense microtubuli arrays in myotubes formed by stacks of differentiated C2C12 myoblasts.
KW  - HIV
KW  - stem-cell
KW  - infection
Y1  - 2013
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-130963
VL  - 8
IS  - 5
ER  -