TY  - JOUR
A1  - Markert, Sebastian Matthias
A1  - Britz, Sebastian
A1  - Proppert, Sven
A1  - Lang, Marietta
A1  - Witvliet, Daniel
A1  - Mulcahy, Ben
A1  - Sauer, Markus
A1  - Zhen, Mei
A1  - Bessereau, Jean-Louis
A1  - Stigloher, Christian
T1  - Filling the gap: adding super-resolution to array tomography for correlated ultrastructural and molecular identification of electrical synapses at the C. elegans connectome
JF  - Neurophotonics
N2  - Correlating molecular labeling at the ultrastructural level with high confidence remains challenging. Array tomography (AT) allows for a combination of fluorescence and electron microscopy (EM) to visualize subcellular protein localization on serial EM sections. Here, we describe an application for AT that combines near-native tissue preservation via high-pressure freezing and freeze substitution with super-resolution light microscopy and high-resolution scanning electron microscopy (SEM) analysis on the same section. We established protocols that combine SEM with structured illumination microscopy (SIM) and direct stochastic optical reconstruction microscopy (dSTORM). We devised a method for easy, precise, and unbiased correlation of EM images and super-resolution imaging data using endogenous cellular landmarks and freely available image processing software. We demonstrate that these methods allow us to identify and label gap junctions in Caenorhabditis elegans with precision and confidence, and imaging of even smaller structures is feasible. With the emergence of connectomics, these methods will allow us to fill in the gap-acquiring the correlated ultrastructural and molecular identity of electrical synapses.
KW  - caenorhabditis elegans
KW  - localization micoscopy
KW  - fluorescent-probes
KW  - junction proteins
KW  - resolution limit
KW  - direct stochasticoptical reconstruction microscopy
KW  - structured illumination microscopy
KW  - correlative light and electron microscopy
KW  - gap junction
KW  - neural circuits
KW  - nervous-system
KW  - image data
KW  - reconstruction
KW  - innexins
KW  - super-resolution microscopy
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-187292
VL  - 3
IS  - 4
ER  - 
TY  - JOUR
A1  - Pauls, Dennis
A1  - Blechschmidt, Christine
A1  - Frantzmann, Felix
A1  - el Jundi, Basil
A1  - Selcho, Mareike
T1  - A comprehensive anatomical map of the peripheral octopaminergic/tyraminergic system of Drosophila melanogaster
JF  - Scientific Reports
N2  - The modulation of an animal’s behavior through external sensory stimuli, previous experience and its internal state is crucial to survive in a constantly changing environment. In most insects, octopamine (OA) and its precursor tyramine (TA) modulate a variety of physiological processes and behaviors by shifting the organism from a relaxed or dormant condition to a responsive, excited and alerted state. Even though OA/TA neurons of the central brain are described on single cell level in Drosophila melanogaster, the periphery was largely omitted from anatomical studies. Given that OA/TA is involved in behaviors like feeding, flying and locomotion, which highly depend on a variety of peripheral organs, it is necessary to study the peripheral connections of these neurons to get a complete picture of the OA/TA circuitry. We here describe the anatomy of this aminergic system in relation to peripheral tissues of the entire fly. OA/TA neurons arborize onto skeletal muscles all over the body and innervate reproductive organs, the heart, the corpora allata, and sensory organs in the antennae, legs, wings and halteres underlining their relevance in modulating complex behaviors.
KW  - neural circuits
KW  - peripheral nervous system
KW  - Drosophila melanogaster
Y1  - 2018
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-177412
VL  - 8
IS  - 15314
ER  -