TY  - JOUR
A1  - Votteler, Miriam
A1  - Carvajal Berrio, Daniel A.
A1  - Pudlas, Marieke
A1  - Walles, Heike
A1  - Schenke-Layland, Katja
T1  - Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
JF  - Journal of Visual Expression
N2  - Non-destructive, non-contact and label-free technologies to monitor cell and tissue cultures are needed in the field of biomedical research.1-5 However, currently available routine methods require processing steps and alter sample integrity. Raman spectroscopy is a fast method that enables the measurement of biological samples without the need for further processing steps. This laser-based technology detects the inelastic scattering of monochromatic light.6 As every chemical vibration is assigned to a specific Raman band (wavenumber in cm-1), each biological sample features a typical spectral pattern due to their inherent biochemical composition.7-9 Within Raman spectra, the peak intensities correlate with the amount of the present molecular bonds.1 Similarities and differences of the spectral data sets can be detected by employing a multivariate analysis (e.g. principal component analysis (PCA)).10

Here, we perform Raman spectroscopy of living cells and native tissues. Cells are either seeded on glass bottom dishes or kept in suspension under normal cell culture conditions (37 °C, 5% CO2) before measurement. Native tissues are dissected and stored in phosphate buffered saline (PBS) at 4 °C prior measurements. Depending on our experimental set up, we then either focused on the cell nucleus or extracellular matrix (ECM) proteins such as elastin and collagen. For all studies, a minimum of 30 cells or 30 random points of interest within the ECM are measured. Data processing steps included background subtraction and normalization.
KW  - tissue engineering
KW  - label-free analysis
KW  - raman spectroscopy
KW  - bioengineering
KW  - living cells
KW  - extracellular matrix
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-124569
VL  - 63
IS  - e3977
ER  - 
TY  - JOUR
A1  - Meyer, Till Jasper
A1  - Gerhard-Hartmann, Elena
A1  - Lodes, Nina
A1  - Scherzad, Agmal
A1  - Hagen, Rudolf
A1  - Steinke, Maria
A1  - Hackenberg, Stephan
T1  - Pilot study on the value of Raman spectroscopy in the entity assignment of salivary gland tumors
JF  - PLoS One
N2  - Background
The entity assignment of salivary gland tumors (SGT) based on histomorphology can be challenging. Raman spectroscopy has been applied to analyze differences in the molecular composition of tissues. The aim of this study was to evaluate the suitability of RS for entity assignment in SGT.

Methods
Raman data were collected in deparaffinized sections of pleomorphic adenomas (PA) and adenoid cystic carcinomas (ACC). Multivariate data and chemometric analysis were completed using the Unscrambler software.

Results
The Raman spectra detected in ACC samples were mostly assigned to nucleic acids, lipids, and amides. In a principal component-based linear discriminant analysis (LDA) 18 of 20 tumor samples were classified correctly.

Conclusion
In this proof of concept study, we show that a reliable SGT diagnosis based on LDA algorithm appears possible, despite variations in the entity-specific mean spectra. However, a standardized workflow for tissue sample preparation, measurement setup, and chemometric algorithms is essential to get reliable results.
KW  - Head and neck cancers
KW  - salivary gland tumors
KW  - salivary glands
KW  - cancers and neoplasms
KW  - malignant tumors
KW  - lipids
KW  - raman spectroscopy
KW  - surgical oncology
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-264736
VL  - 16
IS  - 9
ER  -