TY  - JOUR
A1  - Loeffler, Claudia
A1  - Loeffler, Jürgen
A1  - Kobsar, Anna
A1  - Speer, Christian P.
A1  - Eigenthaler, Martin
T1  - Septic Vs Colonizing Group B Streptococci Differentially Regulate Inflammation and Apoptosis in Human Coronary Artery Endothelial Cells - a Pilot Study
JF  - Journal of Pediatrics and Neonatal Care
N2  - In this pilot study, we exemplify differences between a septic and a colonizing GBS strain during their interaction with Endothelial Cells by evaluating cytokine levels, surface and apoptosis-related molecules. These preliminary results indicate that in vitro infection using an exemplary septic GBS strain results in diminished activation of the innate immune response.
KW  - streptococci
KW  - apoptosis
KW  - inflammation
KW  - endothelial cells
KW  - innate immunity
KW  - early onset sepsis
Y1  - 2015
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-125596
VL  - 2
IS  - 2
ER  - 
TY  - JOUR
A1  - Boeckel, Hannah
A1  - Karsten, Christian M.
A1  - Göpel, Wolfgang
A1  - Herting, Egbert
A1  - Rupp, Jan
A1  - Härtel, Christoph
A1  - Hartz, Annika
T1  - Increased expression of anaphylatoxin C5a-receptor-1 in neutrophils and natural killer cells of preterm infants
JF  - International Journal of Molecular Sciences
N2  - Preterm infants are susceptible to infection and their defense against pathogens relies largely on innate immunity. The role of the complement system for the immunological vulnerability of preterm infants is less understood. Anaphylatoxin C5a and its receptors C5aR1 and -2 are known to be involved in sepsis pathogenesis, with C5aR1 mainly exerting pro-inflammatory effects. Our explorative study aimed to determine age-dependent changes in the expression of C5aR1 and C5aR2 in neonatal immune cell subsets. Via flow cytometry, we analyzed the expression pattern of C5a receptors on immune cells isolated from peripheral blood of preterm infants (n = 32) compared to those of their mothers (n = 25). Term infants and healthy adults served as controls. Preterm infants had a higher intracellular expression of C5aR1 on neutrophils than control individuals. We also found a higher expression of C5aR1 on NK cells, particularly on the cytotoxic CD56\(^{dim}\) subset and the CD56\(^-\) subset. Immune phenotyping of other leukocyte subpopulations revealed no gestational-age-related differences for the expression of and C5aR2. Elevated expression of C5aR1 on neutrophils and NK cells in preterm infants may contribute to the phenomenon of “immunoparalysis” caused by complement activation or to sustained hyper-inflammatory states. Further functional analyses are needed to elucidate the underlying mechanisms.
KW  - preterm infants
KW  - C5a
KW  - C5aR1
KW  - neutrophils
KW  - NK cells
KW  - innate immunity
KW  - sepsis
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-321196
SN  - 1422-0067
VL  - 24
IS  - 12
ER  -