TY  - JOUR
A1  - Jazbutyte, Virginija
A1  - Stumpner, Jan
A1  - Redel, Andreas
A1  - Lorenzen, Johan M.
A1  - Roewer, Norbert
A1  - Thum, Thomas
A1  - Kehl, Franz
T1  - Aromatase Inhibition Attenuates Desflurane-Induced Preconditioning against Acute Myocardial Infarction in Male Mouse Heart In Vivo
JF  - PLoS One
N2  - The volatile anesthetic desflurane (DES) effectively reduces cardiac infarct size following experimental ischemia/reperfusion injury in the mouse heart. We hypothesized that endogenous estrogens play a role as mediators of desflurane-induced preconditioning against myocardial infarction. In this study, we tested the hypothesis that desflurane effects local estrogen synthesis by modulating enzyme aromatase expression and activity in the mouse heart. Aromatase metabolizes testosterone to 17b- estradiol (E2) and thereby significantly contributes to local estrogen synthesis. We tested aromatase effects in acute myocardial infarction model in male mice. The animals were randomized and subjected to four groups which were pre-treated with the selective aromatase inhibitor anastrozole (A group) and DES alone (DES group) or in combination (A+DES group) for 15 minutes prior to surgical intervention whereas the control group received 0.9% NaCl (CON group). All animals were subjected to 45 minutes ischemia following 180 minutes reperfusion. Anastrozole blocked DES induced preconditioning and increased infarct size compared to DES alone (37.94615.5% vs. 17.163.62%) without affecting area at risk and systemic hemodynamic parameters following ischemia/reperfusion. Protein localization studies revealed that aromatase was abundant in the murine cardiovascular system with the highest expression levels in endothelial and smooth muscle cells. Desflurane application at pharmacological concentrations efficiently upregulated aromatase expression in vivo and in vitro. We conclude that desflurane efficiently regulates aromatase expression and activity which might lead to increased local estrogen synthesis and thus preserve cellular integrity and reduce cardiac damage in an acute myocardial infarction model.
KW  - smooth muscle cells
KW  - estrogens
KW  - heart
KW  - anesthetics
KW  - immunostaining
KW  - endothelial cells
KW  - gene expression
KW  - myocardial infarction
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-151258
VL  - 7
IS  - 8
ER  - 
TY  - JOUR
A1  - Hottenrott, Sebastian
A1  - Schlesinger, Tobias
A1  - Helmer, Philipp
A1  - Meybohm, Patrick
A1  - Alkatout, Ibrahim
A1  - Kranke, Peter
T1  - Do small incisions need only minimal anesthesia? — anesthetic management in laparoscopic and robotic surgery
JF  - Journal of Clinical Medicine
N2  - Laparoscopic techniques have established themselves as a major part of modern surgery. Their implementation in every surgical discipline has played a vital part in the reduction of perioperative morbidity and mortality. Precise robotic surgery, as an evolution of this, is shaping the present and future operating theatre that an anesthetist is facing. While incisions get smaller and the impact on the organism seems to dwindle, challenges for anesthetists do not lessen and could even become more demanding than in open procedures. This review focuses on the pathophysiological effects of contemporary laparoscopic and robotic procedures and summarizes anesthetic challenges and strategies for perioperative management.
KW  - general anesthesia
KW  - anesthetics
KW  - perioperative care
KW  - minimally invasive surgery
KW  - laparoscopic surgery
KW  - robotic surgery
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-220039
SN  - 2077-0383
VL  - 9
IS  - 12
ER  -