TY  - JOUR
A1  - Zingler, G.
A1  - Ott, M.
A1  - Blum, G.
A1  - Falkenhagen, U.
A1  - Naumann, G.
A1  - Sokolowska-Köhler, W.
A1  - Hacker, Jörg
T1  - Clonal analysis of Escherichia coli serotype O6 strains from urinary tract infections
N2  - A total of 36 Escherichia coli urinary tract isolates (UTI) of serotype 06, with different combinations of capsule ( K) and flagellin ( H) antigens, were analysed according to the outer membrane pattern (OMP), serum resistance properties, mannose-resistant hemagglutination using various types of erythrocytes, and also for the genetic presence and the expression of Pfimbriae. S fimbriae/F1 C fimbriae, Type 1 fimbriae, aerobactin and hemolysin. Twenty selected strains were further analysed by pulsed field gel electrophoresis (PFGE), elaborating genomic profilas by Xba I cleavage and subsequent Southern hybridization to virulence-associated DNA probes. lt could be shown that 06 UTI isolates represent a highly heterogeneaus group of strains according to the occurrence and combination of these traits. Relatedness an the genetic and the phenotypic Ievei was found for some of the strains exhibiting the same 0: K: H: F serotype. DNA Iang-range mapping further indicated some interesting features, according to the copy number and the genomic linkage of virulence genes.
KW  - Infektionsbiologie
KW  - E. coli serotype 06
KW  - urinary tract infection
KW  - virulence factors
KW  - clonal analysis
KW  - molecular epidemiology
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59786
ER  - 
TY  - JOUR
A1  - Moremi, Nyambura
A1  - Mshana, Stephen E.
A1  - Kamugisha, Erasmus
A1  - Kataraihya, Johannes B.
A1  - Tappe, Dennis
A1  - Vogel, Ulrich
A1  - Lyamuya, Eligius F.
A1  - Claus, Heike
T1  - Predominance of methicillin resistant Staphylococcus aureus-ST88 and new ST1797 causing wound infection and abscesses
JF  - Journal of Infection in Developing Countries
N2  - Introduction: Although there has been a worldwide emergence and spread of methicillin-resistant Staphylococcus aureus (MRSA), little is known about the molecular epidemiology of MRSA in Tanzania. 
Methodology: In this study, we characterized MRSA strains isolated from clinical specimens at the Bugando Medical Centre, Tanzania, between January and December 2008. Of 160 S. aureus isolates from 600 clinical specimens, 24 (15%) were found to be MRSA. Besides molecular screening for the Panton Valentine leukocidin (PVL) genes by PCR, MRSA strains were further characterized by Multi-Locus Sequence Typing (MLST) and spa typing. 
Results: Despite considerable genetic diversity, the spa types t690 (29.1%) and t7231 (41.6%), as well as the sequence types (ST) 88 (54.2%) and 1797 (29.1%), were dominant among clinical isolates. The PVL genes were detected in 4 isolates; of these, 3 were found in ST 88 and one in ST1820. Resistance to erythromycin, clindamicin, gentamicin, tetracycline and co-trimoxazole was found in 45.8%, 62.5%, 41.6%, 45.8% and 50% of the strains, respectively. 
Conclusion: We present the first thorough typing of MRSA at a Tanzanian hospital. Despite considerable genetic diversity, ST88 was dominant among clinical isolates at the Bugando Medical Centre. Active and standardized surveillance of nosocomial MRSA infection should be conducted in the future to analyse the infection and transmission rates and implement effective control measures.
KW  - Tanzania
KW  - panton-valentine leukocidin
KW  - field gel-electrophoreresis
KW  - molecular epidemiology
KW  - aureus infections
KW  - MRSA
KW  - ST88
KW  - ST1797
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-134746
VL  - 6
IS  - 8
ER  -