TY  - JOUR
A1  - Vučićević, Dubravka
A1  - Gehre, Maja
A1  - Dhamija, Sonam
A1  - Friis-Hansen, Lennart
A1  - Meierhofer, David
A1  - Sauer, Sascha
A1  - Ørom, Ulf Andersson
T1  - The long non-coding RNA PARROT is an upstream regulator of c-Myc and affects proliferation and translation
JF  - Oncotarget
N2  - Long non-coding RNAs are important regulators of gene expression and signaling pathways. The expression of long ncRNAs is dysregulated in cancer and other diseases. The identification and characterization of long ncRNAs is often challenging due to their low expression level and localization to chromatin. Here, we identify a functional long ncRNA, PARROT (Proliferation Associated RNA and Regulator Of Translation) transcribed by RNA polymerase II and expressed at a relatively high level in a number of cell lines. The PARROT long ncRNA is associated with proliferation in both transformed and normal cell lines. We characterize the long ncRNA PARROT as an upstream regulator of c-Myc affecting cellular proliferation and translation using RNA sequencing and mass spectrometry following depletion of the long ncRNA. PARROT is repressed during senescence of human mammary epithelial cells and overexpressed in some cancers, suggesting an important association with proliferation through regulation of c-Myc. With this study, we add to the knowledge of cytoplasmic functional long ncRNAs and extent the long ncRNA-Myc regulatory network in transformed and normal cells.
KW  - PARROT
KW  - c-Myc
KW  - long ncRNA
KW  - upstream regulator
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-166519
VL  - 7
IS  - 23
ER  -