TY  - JOUR
A1  - Schwedhelm, Ivo
A1  - Zdzieblo, Daniela
A1  - Appelt-Menzel, Antje
A1  - Berger, Constantin
A1  - Schmitz, Tobias
A1  - Schuldt, Bernhard
A1  - Franke, Andre
A1  - Müller, Franz-Josef
A1  - Pless, Ole
A1  - Schwarz, Thomas
A1  - Wiedemann, Philipp
A1  - Walles, Heike
A1  - Hansmann, Jan
T1  - Automated real-time monitoring of human pluripotent stem cell aggregation in stirred tank reactors
JF  - Scientific Reports
N2  - The culture of human induced pluripotent stem cells (hiPSCs) at large scale becomes feasible with the aid of scalable suspension setups in continuously stirred tank reactors (CSTRs). Innovative monitoring options and emerging automated process control strategies allow for the necessary highly defined culture conditions. Next to standard process characteristics such as oxygen consumption, pH, and metabolite turnover, a reproducible and steady formation of hiPSC aggregates is vital for process scalability. In this regard, we developed a hiPSC-specific suspension culture unit consisting of a fully monitored CSTR system integrated into a custom-designed and fully automated incubator. As a step towards cost-effective hiPSC suspension culture and to pave the way for flexibility at a large scale, we constructed and utilized tailored miniature CSTRs that are largely made from three-dimensional (3D) printed polylactic acid (PLA) filament, which is a low-cost material used in fused deposition modelling. Further, the monitoring tool for hiPSC suspension cultures utilizes in situ microscopic imaging to visualize hiPSC aggregation in real-time to a statistically significant degree while omitting the need for time-intensive sampling. Suitability of our culture unit, especially concerning the developed hiPSC-specific CSTR system, was proven by demonstrating pluripotency of CSTR-cultured hiPSCs at RNA (including PluriTest) and protein level.
KW  - Biomedical engineering
KW  - Stem-cell biotechnology
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-202649
VL  - 9
ER  - 
TY  - JOUR
A1  - Vogel, Patrick
A1  - Markert, Jonathan
A1  - Rückert, Martin A.
A1  - Herz, Stefan
A1  - Keßler, Benedikt
A1  - Dremel, Kilian
A1  - Althoff, Daniel
A1  - Weber, Matthias
A1  - Buzug, Thorsten M.
A1  - Bley, Thorsten A.
A1  - Kullmann, Walter H.
A1  - Hanke, Randolf
A1  - Zabler, Simon
A1  - Behr, Volker C.
T1  - Magnetic Particle Imaging meets computed tomography: first simultaneous imaging
JF  - Scientific Reports
N2  - Magnetic Particle Imaging (MPI) is a promising new tomographic modality for fast as well as three-dimensional visualization of magnetic material. For anatomical or structural information an additional imaging modality such as computed tomography (CT) is required. In this paper, the first hybrid MPI-CT scanner for multimodal imaging providing simultaneous data acquisition is presented.
KW  - Applied physics
KW  - Biomedical engineering
KW  - Imaging techniques
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-202501
VL  - 9
ER  - 
TY  - JOUR
A1  - Salgarella, Alice Rita
A1  - Zahoranová, Anna
A1  - Šrámková, Petra
A1  - Majerčíková, Monika
A1  - Pavlova, Ewa
A1  - Luxenhofer, Robert
A1  - Kronek, Juraj
A1  - Lacík, Igor
A1  - Ricotti, Leonardo
T1  - Investigation of drug release modulation from poly(2-oxazoline) micelles through ultrasound
JF  - Scientific Reports
N2  - Among external stimuli used to trigger release of a drug from a polymeric carrier, ultrasound has gained increasing attention due to its non-invasive nature, safety and low cost. Despite this attention, there is only limited knowledge about how materials available for the preparation of drug carriers respond to ultrasound. This study investigates the effect of ultrasound on the release of a hydrophobic drug, dexamethasone, from poly(2-oxazoline)-based micelles. Spontaneous and ultrasound-mediated release of dexamethasone from five types of micelles made of poly(2-oxazoline) block copolymers, composed of hydrophilic poly(2-methyl-2-oxazoline) and hydrophobic poly(2-n-propyl-2-oxazoline) or poly(2-butyl-2-oxazoline-co-2-(3-butenyl)-2-oxazoline), was studied. The release profiles were fitted by zeroorder and Ritger-Peppas models. The ultrasound increased the amount of released dexamethasone by 6% to 105% depending on the type of copolymer, the amount of loaded dexamethasone, and the stimulation time point. This study investigates for the first time the interaction between different poly(2-oxazoline)-based micelle formulations and ultrasound waves, quantifying the efficacy of such stimulation in modulating dexamethasone release from these nanocarriers.
KW  - Acoustics
KW  - Biomedical engineering
KW  - Drug delivery
KW  - Materials chemistry
Y1  - 2018
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-227277
VL  - 8
ER  -