TY  - JOUR
A1  - Wagner-Drouet, Eva
A1  - Teschner, Daniel
A1  - Wolschke, Christine
A1  - Schäfer-Eckart, Kerstin
A1  - Gärtner, Johannes
A1  - Mielke, Stephan
A1  - Schreder, Martin
A1  - Kobbe, Guido
A1  - Hilgendorf, Inken
A1  - Klein, Stefan
A1  - Verbeek, Mareike
A1  - Ditschkowski, Markus
A1  - Koch, Martina
A1  - Lindemann, Monika
A1  - Schmidt, Traudel
A1  - Rascle, Anne
A1  - Barabas, Sascha
A1  - Deml, Ludwig
A1  - Wagner, Ralf
A1  - Wolff, Daniel
T1  - Comparison of cytomegalovirus-specific immune cell response to proteins versus peptides using an IFN-γ ELISpot assay after hematopoietic stem cell transplantation
JF  - Diagnostics
N2  - Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality following hematopoietic stem cell transplantation (HSCT). Measuring CMV-specific cellular immunity may improve the risk stratification and management of patients. IFN-γ ELISpot assays, based on the stimulation of peripheral blood mononuclear cells with CMV pp65 and IE-1 proteins or peptides, have been validated in clinical settings. However, it remains unclear to which extend the T-cell response to synthetic peptides reflect that mediated by full-length proteins processed by antigen-presenting cells. We compared the stimulating ability of pp65 and IE-1 proteins and corresponding overlapping peptides in 16 HSCT recipients using a standardized IFN-γ ELISpot assay. Paired qualitative test results showed an overall 74.4% concordance. Discordant results were mainly due to low-response tests, with one exception. One patient with early CMV reactivation and graft-versus-host disease, sustained CMV DNAemia and high CD8\(^+\) counts showed successive negative protein-based ELISpot results but a high and sustained response to IE-1 peptides. Our results suggest that the response to exogenous proteins, which involves their uptake and processing by antigen-presenting cells, more closely reflects the physiological response to CMV infection, while the response to exogenous peptides may lead to artificial in vitro T-cell responses, especially in strongly immunosuppressed patients.
KW  - CMV
KW  - CMV-specific cellular immunity
KW  - hematopoietic stem cell transplantation
KW  - recall antigen
KW  - peptide
KW  - immune monitoring
KW  - IFN-γ ELISpot
KW  - T cells
KW  - antigen processing and presentation
KW  - immunosuppression
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-228843
SN  - 2075-4418
VL  - 11
IS  - 2
ER  - 
TY  - JOUR
A1  - Luber, Verena
A1  - Lutz, Mathias
A1  - Abele-Horn, Marianne
A1  - Einsele, Hermann
A1  - Grigoleit, Götz Ulrich
A1  - Mielke, Stephan
T1  - Excretion of Ascaris lumbricoides following reduced‐intensity allogeneic hematopoietic stem cell transplantation and consecutive treatment with mebendazole
JF  - Transplant Infectious Disease
N2  - Here, we present the unique case of a 51‐year‐old German patient with multiple myeloma excreting Ascaris lumbricoides in his stool five weeks after allogeneic hematopoietic stem cell transplantation. Stool analysis remained negative for the presence of eggs, and there was no eosinophilia in the peripheral blood at any time around stem cell transplantation. The patient was commenced on a three‐day treatment with mebendazole, which was well tolerated. No serious interactions with the concomitant post‐transplant medication or negative effects on the hematopoiesis were observed, and the myeloma still is in complete remission. To our knowledge, this is the first report on excretion of A lumbricoides in the context of allogeneic stem cell transplantation. The case is remarkable with view to the fact that the parasite has supposedly survived all courses of myeloma treatment including autologous and allogeneic conditioning. Parasitosis with A lumbricoides has a worldwide prevalence of about a billion and is extremely rare in northern Europe. Possibly the patient got infected during a trip to Egypt years before multiple myeloma was diagnosed.
KW  - sirolimus
KW  - mycophenolic acid
KW  - multiple myeloma
KW  - mebendazole
KW  - hematopoietic stem cell transplantation
KW  - Ascaris lumbricoides
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-219608
SN  - 1399-3062
VL  - 22
IS  - 1
ER  -