TY  - JOUR
A1  - Bender, L.
A1  - Ott, M.
A1  - Marre, R.
A1  - Hacker, Jörg
T1  - Genome analysis of Legionella spp. by orthogonal field alternation gel electrophoresis (OFAGE)
N2  - Various Legionella isolates from different sources and origins were analysed by orthogonal field alternation gel electrophoresis of Not I cleaved genomic DNA. The genome of L pneumophila Philadelphia I, the original isolate of the epidemics in 1976, exhibits only five Not I fragments. Two virulent derivatives. derived from L pneumophila Philadelphia I. which were obtained by prolonged passage on artificial cuhure media, did not differ from their isogenic virulent strain according the Not I fragment pattern. By summing the lengths of the Notl fragments, the genome size of L. pneumophila Philadelphia I was calculated as approximately 3.9 Mb. Environmental L pneumophila strains exhibited different Not I pattems, as did Legionella strains not belongi'ng to the species pneumophila. The usefulness of DNA long range mapping of Legionella ssp. with Notl for epidemiology and evaluation of their evolutionary rela· tionships is discussed.
KW  - Infektionsbiologie
KW  - Legionella ssp.
KW  - Genome analysis
KW  - Orthogonal field attenuation gel electrophoresis
Y1  - 1990
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59657
ER  - 
TY  - JOUR
A1  - Hacker, Jörg
A1  - Bender, L.
A1  - Ott, M.
A1  - Wingeder, J.
A1  - Lund, B.
A1  - Marre, R.
A1  - Goebel, W.
T1  - Deletions of chromosomal regions coding for fimbriae and hemolysins occur in vivo and in vitro in various extraintestinal Escherichia coli isolates
N2  - No abstract available
KW  - Infektionsbiologie
KW  - P-fimbriae
KW  - hemolysin
KW  - genomic deletions
KW  - extraintestinal E. coli
KW  - virulence modulation
Y1  - 1990
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59608
ER  - 
TY  - JOUR
A1  - Marre, R.
A1  - Kreft, B.
A1  - Hacker, Jörg
T1  - Genetically engineered S and F1C fimbriae differ in their contribution to adherence of Escherichia coli to cultured renal tubular cells
N2  - Escherichia coU K-12 strains producing S-fimbrial adhesins, FlC fimbriae, and mutagenized fimbriae were tested in a binding assay with a renal tubular cell line. S-fimbrial adhesins and FlC fimbriae mediated bindlog to tubular cells. The SfaA, SfaG, and SfaS subunits of S fimbriae contributed to attachment. Site-specific mutations in the sfaS gene reduced binding. The Inhibitionprofile of FlC fimbriae resembled that of S fimbriae.
KW  - Infektionsbiologie
Y1  - 1990
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59644
ER  -