TY  - JOUR
A1  - Wegener, Christian
A1  - Karsai, Gergely
A1  - Pollák, Edit
A1  - Wacker, Matthias
A1  - Vömel, Matthias
A1  - Selcho, Mareike
A1  - Berta, Gergely
A1  - Nachman, Ronald J.
A1  - Isaac, R. Elwyn
A1  - Molnár, László
T1  - Diverse in- and output polarities and high complexity of local synaptic and non-synaptic signaling within a chemically defined class of peptidergic Drosophila neurons
JF  - Frontiers in Neural Circuits
N2  - Peptidergic neurons are not easily integrated into current connectomics concepts, since their peptide messages can be distributed via non-synaptic paracrine signaling or volume transmission. Moreover, the polarity of peptidergic interneurons in terms of in- and out-put sites can be hard to predict and is very little explored. We describe in detail the morphology and the subcellular distribution of fluorescent vesicle/dendrite markers in CCAP neurons (NCCAP), a well defined set of peptidergic neurons in the Drosophila larva. NCCAP can be divided into five morphologically distinct subsets. In contrast to other subsets, serial homologous interneurons in the ventral ganglion show a mixed localization of in- and output markers along ventral neurites that defy a classification as dendritic or axonal compartments. Ultrastructurally, these neurites contain both pre- and postsynaptic sites preferably at varicosities. A significant portion of the synaptic events are due to reciprocal synapses. Peptides are mostly non-synaptically or parasynaptically released, and dense-core vesicles and synaptic vesicle pools are typically well separated. The responsiveness of the NCCAP to ecdysis-triggering hormone may be at least partly dependent on a tonic synaptic inhibition, and is independent of ecdysteroids. Our results reveal a remarkable variety and complexity of local synaptic circuitry within a chemically defined set of peptidergic neurons. Synaptic transmitter signaling as well as peptidergic paracrine signaling and volume transmission from varicosities can be main signaling modes of peptidergic interneurons depending on the subcellular region. The possibility of region-specific variable signaling modes should be taken into account in connectomic studies that aim to dissect the circuitry underlying insect behavior and physiology, in which peptidergic neurons act as important regulators.
KW  - synaptic signaling
KW  - volume transmission
KW  - paracrine release
KW  - neuromodulation
KW  - ecdysis
KW  - bursicon
KW  - CCAP
KW  - myoinhibitory peptide
Y1  - 2013
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-96914
ER  - 
TY  - JOUR
A1  - Buchner, Erich
A1  - Blanco Redondo, Beatriz
A1  - Bunz, Melanie
A1  - Halder, Partho
A1  - Sadanandappa, Madhumala K.
A1  - Mühlbauer, Barbara
A1  - Erwin, Felix
A1  - Hofbauer, Alois
A1  - Rodrigues, Veronica
A1  - VijayRaghavan, K.
A1  - Ramaswami, Mani
A1  - Rieger, Dirk
A1  - Wegener, Christian
A1  - Förster, Charlotte
T1  - Identification and Structural Characterization of Interneurons of the Drosophila Brain by Monoclonal Antibodies of the Würzburg Hybridoma Library
JF  - PLoS ONE
N2  - Several novel synaptic proteins have been identified by monoclonal antibodies (mAbs) of the Würzburg hybridoma library generated against homogenized Drosophila brains, e.g. cysteine string protein, synapse-associated protein of 47 kDa, and Bruchpilot. However, at present no routine technique exists to identify the antigens of mAbs of our library that label only a small number of cells in the brain. Yet these antibodies can be used to reproducibly label and thereby identify these cells by immunohistochemical staining. Here we describe the staining patterns in the Drosophila brain for ten mAbs of the Würzburg hybridoma library. Besides revealing the neuroanatomical structure and distribution of ten different sets of cells we compare the staining patterns with those of antibodies against known antigens and GFP expression patterns driven by selected Gal4 lines employing regulatory sequences of neuronal genes. We present examples where our antibodies apparently stain the same cells in different Gal4 lines suggesting that the corresponding regulatory sequences can be exploited by the split-Gal4 technique for transgene expression exclusively in these cells. The detection of Gal4 expression in cells labeled by mAbs may also help in the identification of the antigens recognized by the antibodies which then in addition to their value for neuroanatomy will represent important tools for the characterization of the antigens. Implications and future strategies for the identification of the antigens are discussed.
KW  - cell staining
KW  - drosophila melanogaster
KW  - gene expression
KW  - hybridomas
KW  - immune serum
KW  - library screening
KW  - monoclonal antibodies
KW  - neurons
Y1  - 2013
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-97109
ER  -