TY  - JOUR
A1  - Ruppert, Manuela
A1  - Franz, Mirjam
A1  - Saratis, Anastasios
A1  - Escarcena, Laura Velo
A1  - Hendrich, Oliver
A1  - Gooi, Li Ming
A1  - Schwenkert, Isabell
A1  - Klebes, Ansgar
A1  - Scholz, Henrike
T1  - Hangover links nuclear RNA signaling to cAMP regulation via the phosphodiesterase 4d ortholog dunce
JF  - Cell Reports
N2  - The hangover gene defines a cellular stress pathway that is required for rapid ethanol tolerance in Drosophila melanogaster. To understand how cellular stress changes neuronal function, we analyzed Hangover function on a cellular and neuronal level. We provide evidence that Hangover acts as a nuclear RNA binding protein and we identified the phosphodiesterase 4d ortholog dunce as a target RNA. We generated a transcript-specific dunce mutant that is impaired not only in ethanol tolerance but also in the cellular stress response. At the neuronal level, Dunce and Hangover are required in the same neuron pair to regulate experience-dependent motor output. Within these neurons, two cyclic AMP (cAMP)-dependent mechanisms balance the degree of tolerance. The balance is achieved by feedback regulation of Hangover and dunce transcript levels. This study provides insight into how nuclear Hangover/RNA signaling is linked to the cytoplasmic regulation of cAMP levels and results in neuronal adaptation and behavioral changes.
KW  - biology
KW  - hangover
KW  - dunce
KW  - Dunce isoforms
KW  - PDE4d
KW  - cellular stress
KW  - alcohol tolerance
KW  - Drosophila melanogaster
Y1  - 2017
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-171950
VL  - 18
IS  - 2
ER  - 
TY  - JOUR
A1  - Xu, Li
A1  - He, Jianzheng
A1  - Kaiser, Andrea
A1  - Gräber, Nikolas
A1  - Schläger, Laura
A1  - Ritze, Yvonne
A1  - Scholz, Henrike
T1  - A Single Pair of Serotonergic Neurons Counteracts Serotonergic Inhibition of Ethanol Attraction in Drosophila
JF  - PLoS ONE
N2  - Attraction to ethanol is common in both flies and humans, but the neuromodulatory mechanisms underlying this innate attraction are not well understood. Here, we dissect the function of the key regulator of serotonin signaling—the serotonin transporter–in innate olfactory attraction to ethanol in Drosophila melanogaster. We generated a mutated version of the serotonin transporter that prolongs serotonin signaling in the synaptic cleft and is targeted via the Gal4 system to different sets of serotonergic neurons. We identified four serotonergic neurons that inhibit the olfactory attraction to ethanol and two additional neurons that counteract this inhibition by strengthening olfactory information. Our results reveal that compensation can occur on the circuit level and that serotonin has a bidirectional function in modulating the innate attraction to ethanol. Given the evolutionarily conserved nature of the serotonin transporter and serotonin, the bidirectional serotonergic mechanisms delineate a basic principle for how random behavior is switched into targeted approach behavior.
KW  - attraction
KW  - ethanol
KW  - Drosophila melanogaster
KW  - serotonin transporter
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-166762
VL  - 11
IS  - 12
ER  - 
TY  - JOUR
A1  - Aso, Yoshinori
A1  - Herb, Andrea
A1  - Ogueta, Maite
A1  - Siwanowicz, Igor
A1  - Templier, Thomas
A1  - Friedrich, Anja B.
A1  - Ito, Kei
A1  - Scholz, Henrike
A1  - Tanimoto, Hiromu
T1  - Three Dopamine Pathways Induce Aversive Odor Memories with Different Stability
JF  - PLoS Genetics
N2  - Animals acquire predictive values of sensory stimuli through reinforcement. In the brain of Drosophila melanogaster, activation of two types of dopamine neurons in the PAM and PPL1 clusters has been shown to induce aversive odor memory. Here, we identified the third cell type and characterized aversive memories induced by these dopamine neurons. These three dopamine pathways all project to the mushroom body but terminate in the spatially segregated subdomains. To understand the functional difference of these dopamine pathways in electric shock reinforcement, we blocked each one of them during memory acquisition. We found that all three pathways partially contribute to electric shock memory. Notably, the memories mediated by these neurons differed in temporal stability. Furthermore, combinatorial activation of two of these pathways revealed significant interaction of individual memory components rather than their simple summation. These results cast light on a cellular mechanism by which a noxious event induces different dopamine signals to a single brain structure to synthesize an aversive memory.
KW  - dynamics
KW  - serotonin
KW  - expression
KW  - melanogaster
KW  - neurons form
KW  - olfactory memory
KW  - long-term-memory
KW  - drosophila mushroom body
KW  - sensitization
KW  - localization
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-130631
VL  - 8
IS  - 7
ER  -