TY  - JOUR
A1  - Karakaya, Emine
A1  - Bider, Faina
A1  - Frank, Andreas
A1  - Teßmar, Jörg
A1  - Schöbel, Lisa
A1  - Forster, Leonard
A1  - Schrüfer, Stefan
A1  - Schmidt, Hans-Werner
A1  - Schubert, Dirk Wolfram
A1  - Blaeser, Andreas
A1  - Boccaccini, Aldo R.
A1  - Detsch, Rainer
T1  - Targeted printing of cells: evaluation of ADA-PEG bioinks for drop on demand approaches
JF  - Gels
N2  - A novel approach, in the context of bioprinting, is the targeted printing of a defined number of cells at desired positions in predefined locations, which thereby opens up new perspectives for life science engineering. One major challenge in this application is to realize the targeted printing of cells onto a gel substrate with high cell survival rates in advanced bioinks. For this purpose, different alginate-dialdehyde—polyethylene glycol (ADA-PEG) inks with different PEG modifications and chain lengths (1–8 kDa) were characterized to evaluate their application as bioinks for drop on demand (DoD) printing. The biochemical properties of the inks, printing process, NIH/3T3 fibroblast cell distribution within a droplet and shear forces during printing were analyzed. Finally, different hydrogels were evaluated as a printing substrate. By analysing different PEG chain lengths with covalently crosslinked and non-crosslinked ADA-PEG inks, it was shown that the influence of Schiff's bases on the viscosity of the corresponding materials is very low. Furthermore, it was shown that longer polymer chains resulted in less stable hydrogels, leading to fast degradation rates. Several bioinks highly exhibit biocompatibility, while the calculated nozzle shear stress increased from approx. 1.3 and 2.3 kPa. Moreover, we determined the number of cells for printed droplets depending on the initial cell concentration, which is crucially needed for targeted cell printing approaches.
KW  - bioprinting
KW  - drop on demand
KW  - sodium alginate
KW  - polyethylene glycol
KW  - shear stress
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-267317
SN  - 2310-2861
VL  - 8
IS  - 4
ER  - 
TY  - JOUR
A1  - Jarausch, Johannes
A1  - Neuenroth, Lisa
A1  - Andag, Reiner
A1  - Leha, Andreas
A1  - Fischer, Andreas
A1  - Asif, Abdul R.
A1  - Lenz, Christof
A1  - Eidizadeh, Abass
T1  - Influence of shear stress, inflammation and BRD4 inhibition on human endothelial cells: a holistic proteomic approach
JF  - Cells
N2  - Atherosclerosis is an important risk factor in the development of cardiovascular diseases. In addition to increased plasma lipid concentrations, irregular/oscillatory shear stress and inflammatory processes trigger atherosclerosis. Inhibitors of the transcription modulatory bromo- and extra-terminal domain (BET) protein family (BETi) could offer a possible therapeutic approach due to their epigenetic mechanism and anti-inflammatory properties. In this study, the influence of laminar shear stress, inflammation and BETi treatment on human endothelial cells was investigated using global protein expression profiling by ion mobility separation-enhanced data independent acquisition mass spectrometry (IMS-DIA-MS). For this purpose, primary human umbilical cord derived vascular endothelial cells were treated with TNFα to mimic inflammation and exposed to laminar shear stress in the presence or absence of the BRD4 inhibitor JQ1. IMS-DIA-MS detected over 4037 proteins expressed in endothelial cells. Inflammation, shear stress and BETi led to pronounced changes in protein expression patterns with JQ1 having the greatest effect. To our knowledge, this is the first proteomics study on primary endothelial cells, which provides an extensive database for the effects of shear stress, inflammation and BETi on the endothelial proteome.
KW  - HUVEC
KW  - shear stress
KW  - endothelial
KW  - proteomic
KW  - BRD4
KW  - JQ1
KW  - DIA-MS
KW  - BET Inhibitor
KW  - atherosclerosis
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-289872
SN  - 2073-4409
VL  - 11
IS  - 19
ER  -