TY - JOUR A1 - Gillitzer, Reinhard A1 - Berger, Rudolf A1 - Moll, Heidrun T1 - A reliable method for simultaneous demonstration of two antigens using a novel combination of immunogold-silver staining with immunoenzymatic labeling N2 - We have developed a reliable and sensitive immunohistochemical staining technique which allows the simultaneous demonstration of two different antigens expressed in or on the same cell (referred to as mixed labeling), together with the evaluation of the general histopathological appearance of the tissue. The staining procedure combines a three-step (streptavidin-biotin) immunogold-silver staining (IGSS) with a three-step immunoenzymatic labeling. For this purpose, we investigated the compatibility ofIGSS with various substrates of peroxidase or alkaline phosphatase (AP). Highly reliable and discernible mixed labeling was achieved only after iniriallabeling with IGSS followed by AP labeling using the substrates naphthol AS-MX phosphate/Fast Blue or naphthol AS-HI phosphate/New Fuchsin, respectively. To ensure utmost specificity, we applied FlTC-conjugated mouse monoclonal antibodies and rabbit anti-FlTC immunoglobulins visualized by AP-labeled immunoglobulins and the respective substrate in a final step. This novel approach provides an excellent means for demonstration of immunocompetent cells and unequivocal determination of the percentage of specific cell subsets in infiltrated tissue. The advantages of this method, as compared with double immunofluorescence or double immunoenzymatic labeling, were investigated and are discussed. (J Histochem Cytochem 38:307-313, 1990) KW - Immunohistochemistry; Immunogold-silver staining; FITC- anti-FITC system; Leucocyte subpopulations; Two-color staining Y1 - 1990 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-31092 ER - TY - JOUR A1 - Parodi, S. A1 - Lutz, Werner K. A1 - Colacci, A. A1 - Mazzullo, M. A1 - Taningher, M. A1 - Grilli, S. T1 - Results of animal studies suggest a nonlinear dose-response relationship for benzene effects N2 - Considering the very large industrial usage of benzene, studies in risk assessment aimed at the evaluation of carcinogenic risk at low Ievels of exposure are important. Animal data can offer indications about what could happen in humans and provide more diverse information than epidemiological data with respect to doseresponse consideration. We have considered experiments investigating metabolism, short·term genotoxicity tests, DNA adduct formation, and carcinogenicity long-term tests. According to the different experiments, a Saturation of benzene metabolism and benzene effects in terms of genotoxicity seems evident above 30 to 100 ppm. Below 30 to 60 ppm the initiating effect ofbenzene seems tobe linear fora large intervaJ ofdosages, at least judging from DNA adduct formation. Potentiallack of a promoting effect of benzene (below 10 ppm) could generate a sublinear response at nontox.ic levels of ex.posure. This possibility was suggested by epidemiological data in humans and is not confirmed or excluded by our observations with animals. KW - Toxikologie Y1 - 1989 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-60843 ER - TY - JOUR A1 - Hünig, Siegfried A1 - Wehner, Ingeborg T1 - A convenient synthesis of 2,2',6,6'-Tetramethyl-4,4'-bipyridine and its oxidation to 2,2',6,6'-Tetracarboxy-4,4'-bipyridine N2 - No abstract available Y1 - 1989 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-32005 ER - TY - JOUR A1 - Schneider, Wolfgang T1 - Book Reviews: Cognition, Metacognition, and Reading N2 - No abstract available KW - Psychologie Y1 - 1988 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-62079 ER - TY - JOUR A1 - Hommers, Wilfried T1 - Review of "Roskam, E.E., & Suck, R. (Eds.): Progress in mathematical psychology. Amsterdam: North-Holland, 1987, pp. 538." N2 - No abstract available Y1 - 1988 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-43525 ER - TY - JOUR A1 - Feuerstein, G. A1 - Sirén, Anna-Leena T1 - Opioid peptides: A role in hypertension? [Brief Review] N2 - This review is an attempt to highlight evidence that may implicate the endogenaus opioid system in the pathogenesis of hypertension in humans. The evidence raised includes biochemical, physiological, pharmacological, and behavioral studies con~ucted in in vitro andin vivo systems, experimental models of hypertension, and hornans with essential hypertension. While the compelling biochemical and pharmacological evidence in experimental animals clearly shows the presence of opioid peptides and their receptors in strategic sites of cardiovascular control and potent cardiovascular response to opioid peptides, opioid antagonists show no consistent blockade or reversal of hypertension in experimental animals or humans. One possible explanation for this phenomenon could be the vast redundancy in systems regulating blood pressure (i.e., the blockade ofone system stillleaves many other systerils fully able to rapidly offset the eliminated system). Regarding the opioid system, the situation is much more complex, since some opioid receptors (\(\mu\)-type) niediate pressor responses, while other receptors (\(\kappa\)type) mediate depressor responses. Therefore, nonselective opioid receptor antagonists (e.g., naloxone), which block both types ofreceptors, can be devoid ofany cardiovascular activity, while a selective \(\mu\)-receptor antagonist or a selective arid potent \(\kappa\)-receptor agonist may produce the desired antihypertensive elfect. A combination of both actions (i.e., a drug that is both \(\mu\)antagonist and a \(\kappa\)antagonist) might be even more advantageous. Until such compounds are developed, this hypothesis will be hard to prove. KW - Neurobiologie Y1 - 1987 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63262 ER - TY - JOUR A1 - Reimer, Georg A1 - Raska, Ivan A1 - Tan, Eng M. A1 - Scheer, Ulrich T1 - Human autoantibodies: probes for nucleolus structure and function N2 - No abstract available Y1 - 1987 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-41410 ER - TY - JOUR A1 - Schneider, Wolfgang T1 - How to avoid traps and fallacies: The multilevel issue in educational research N2 - Review of "Hans Oosthoek and Pieter van den Eeden (Eds.) Education From the Multi-Level Perspective: Models, Methodology and Empirical Findings. - London: Gordon & Breach Science, 1984. 295 pp." Y1 - 1986 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-87336 ER - TY - JOUR A1 - Ellgring, Johann Heinrich T1 - Nonverbal communication - A review of research in Germany N2 - This paper presents an overview of the research on nonverbal communication that has appeared in the German-language literature during the past decade, and gives some treatment of its relationship to Ausdruckspsychologie. Empirical studies, recent theoretical issues, and methodological developments are discussed. - Although nonverbal communication often plays an essential role in diagnosis and treatment, it has been widely neglected in academic training for the past 20 years. This inconsistency may partly be due to the outright rejection of the classical Ausdruckspsychologie during the 1960's. In order to avoid the fate of Ausdruckspsychologie, it will be necessary to extend our knowledge of nonverbal communication by means of further methodological development and empirical investigation Y1 - 1981 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-42022 ER - TY - JOUR A1 - Ellgring, Johann Heinrich T1 - Comment to J. Rojahn: "Validity and reliability of data from naturalistic observational studies - problems and alternatives" N2 - No abstract available Y1 - 1978 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-42520 ER -