TY  - JOUR
A1  - Geisinger, Adriana
A1  - Rodríguez-Casuriaga, Rosana
A1  - Benavente, Ricardo
T1  - Transcriptomics of Meiosis in the Male Mouse
JF  - Frontiers in Cell and Developmental Biology
N2  - Molecular studies of meiosis in mammals have been long relegated due to some intrinsic obstacles, namely the impossibility to reproduce the process in vitro, and the difficulty to obtain highly pure isolated cells of the different meiotic stages. In the recent years, some technical advances, from the improvement of flow cytometry sorting protocols to single-cell RNAseq, are enabling to profile the transcriptome and its fluctuations along the meiotic process. In this mini-review we will outline the diverse methodological approaches that have been employed, and some of the main findings that have started to arise from these studies. As for practical reasons most studies have been carried out in males, and mostly using mouse as a model, our focus will be on murine male meiosis, although also including specific comments about humans. Particularly, we will center on the controversy about gene expression during early meiotic prophase; the widespread existing gap between transcription and translation in meiotic cells; the expression patterns and potential roles of meiotic long non-coding RNAs; and the visualization of meiotic sex chromosome inactivation from the RNAseq perspective.
KW  - meiosis
KW  - transcriptomics
KW  - RNAseq
KW  - meiotic prophase
KW  - spermatogenesis
KW  - lncRNAs
KW  - MSCI
KW  - spermatogenic cell sorting
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-231032
SN  - 2296-634X
VL  - 9
ER  - 
TY  - JOUR
A1  - Kunz, Meik
A1  - Wolf, Beat
A1  - Schulze, Harald
A1  - Atlan, David
A1  - Walles, Thorsten
A1  - Walles, Heike
A1  - Dandekar, Thomas
T1  - Non-Coding RNAs in Lung Cancer: Contribution of Bioinformatics Analysis to the Development of Non-Invasive Diagnostic Tools
JF  - Genes
N2  - Lung cancer is currently the leading cause of cancer related mortality due to late diagnosis and limited treatment intervention. Non-coding RNAs are not translated into proteins and have emerged as fundamental regulators of gene expression. Recent studies reported that microRNAs and long non-coding RNAs are involved in lung cancer development and progression. Moreover, they appear as new promising non-invasive biomarkers for early lung cancer diagnosis. Here, we highlight their potential as biomarker in lung cancer and present how bioinformatics can contribute to the development of non-invasive diagnostic tools. For this, we discuss several bioinformatics algorithms and software tools for a comprehensive understanding and functional characterization of microRNAs and long non-coding RNAs.
KW  - lung cancer
KW  - non-invasive biomarkers
KW  - miRNAs
KW  - lncRNAs
KW  - bioinformatics
KW  - early diagnosis
KW  - algorithm
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-147990
VL  - 8
IS  - 1
ER  -