TY - BOOK A1 - Wilhelm, Gernot T1 - The Hurrians / [with a chapter by D. L. Stein] N2 - No abstract available. KW - Mesopotamien KW - Churriter KW - Mesopotamia KW - Hurrians Y1 - 1989 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-87615 ER - TY - BOOK A1 - Wang, Wen T1 - Validation of shRNA clones for gene silencing in 293FT cells N2 - ... N2 - The goal of the project was to establish knock down of mRNA in human mesenchymal stem cells. Since these cells are difficult to transfect, a viral approach is needed to achieve sufficient expression of e. g. shRNA in a high percentage of cells to allow for an efficient silencing of corresponding mRNAs. For this purpose for every gene product of interest, a number of shRNA clones have to be tested to detect an individual shRNA with sufficient efficacy. Lentiviral systems for shRNA approaches have recently become available. The principal advantage of the lentiviral system is that it allows gene silencing in nondividing cells and therefore expands the usefulness of the RNAi-based gene silencing system. Lentivirus-delivered shRNAs are capable of specific, highly stable and functional silencing of gene expression in a variety of cell types. Since the viral transfection of MSCs is a time consuming process that involves transfection of 293 FT cells plus transduction of target cells, for this thesis the following approach was chosen: genes of interest were checked for expression in 293FT cells by RT-PCR. These gene products can be silenced in 293FT cells simply by transfection of shRNA clones and efficacy was subsequently tested by RT-PCR. Beyond this thesis then the project can proceed with effective clones to transduce primary MSCs with individual shRNA clones identified as effective silencing tool in this thesis. KW - shRNA KW - RNAi KW - .................................................................... KW - shRNA KW - RNAi Y1 - 2008 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-25955 N1 - Aus rechtlichen Gründen wurde der Zugriff auf den Volltext zu diesem Dokument gesperrt. ER - TY - BOOK A1 - Tran-Gia, Phuoc A1 - Hoßfeld, Tobias T1 - Performance Modeling and Analysis of Communication Networks BT - A Lecture Note N2 - This textbook provides an introduction to common methods of performance modeling and analysis of communication systems. These methods form the basis of traffic engineering, teletraffic theory, and analytical system dimensioning. The fundamentals of probability theory, stochastic processes, Markov processes, and embedded Markov chains are presented. Basic queueing models are described with applications in communication networks. Advanced methods are presented that have been frequently used in recent practice, especially discrete-time analysis algorithms, or which go beyond classical performance measures such as Quality of Experience or energy efficiency. Recent examples of modern communication networks include Software Defined Networking and the Internet of Things. Throughout the book, illustrative examples are used to provide practical experience in performance modeling and analysis. Target group: The book is aimed at students and scientists in computer science and technical computer science, operations research, electrical engineering and economics. N2 - Dieses Lehrbuch bietet eine Einführung in gängige Methoden zur Modellbildung und analytische Leistungsbewertung von Kommunikationssystemen. Diese Methoden bilden die Grundlage für Verkehrstheorie und Systemdimensionierung. Die Grundlagen der Wahrscheinlichkeitstheorie, stochastische Prozesse, Markov-Prozesse und eingebettete Markov-Ketten werden vorgestellt. Grundlegende Warteschlangenmodelle werden mit Anwendungen aus Kommunikationsnetzwerken beschrieben. Es werden auch weiterführende Methoden vorgestellt, die in der jüngeren Praxis häufig verwendet wurden, insbesondere zeitdiskrete Analysealgorithmen, oder QoE und Energieeffizienz. Aktuelle Beispiele für moderne Kommunikationsnetze sind Software Defined Networking oder das Internet der Dinge. Im gesamten Buch werden anschauliche Beispiele verwendet, um praktische Erfahrungen in der Leistungsmodellierung und -analyse zu vermitteln. Zielgruppe: Das Buch richtet sich an Studierende und WissenschaftlerInnen aus den Bereichen Informatik und technische Informatik, Operations Research, Elektrotechnik und Wirtschaftswissenschaft. KW - performance modeling KW - Markovian and Non-Markovian systems KW - discrete-time models and analysis KW - communication networks KW - communication network KW - performance evaluation KW - Markov model KW - stochastic processes KW - queueing theory Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-241920 SN - 978-3-95826-152-5 SN - 978-3-95826-153-2 N1 - Parallel erschienen als Druckausgabe in Würzburg University Press, 978-3-95826-152-5, 65,00 Euro. PB - Würzburg University Press CY - Würzburg ET - 1st edition ER - TY - BOOK A1 - Strack, Fritz A1 - Argyle, Michael A1 - Schwarz, Norbert T1 - Subjective well-being : an interdisciplinary perspective N2 - This volume brings together several authors from different areas of psychology and the neighbouring social sciences. Each one contributes their own perspective on the growing interest topic of subjective well-being. The aim of the volume is to present these divergent perspectives and to foster communication between the different areas. Split into three parts, this volume initially discusses the general perspectives of subjective well-being and addresses fundamental questions, secondly it discusses the dynamics of subjective well-being and more specific research issues to give a better understanding of the general phenomenon, and thirdly the book emphasizes the social context in which people experience and report their happiness and satisfaction. The book will be of great interest to social and clinical psychologists, students of psychology and sociology and health professionals. KW - Wohlbefinden KW - Glück KW - Wohlbefinden KW - Glück KW - Well-being KW - Happiness Y1 - 2007 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-21703 ER - TY - BOOK A1 - Smith Pasqualini, Marcia A1 - Macht, Michael A1 - Ellgring, Heiner T1 - Cognitive Behavioral Therapy for People with Parkinson’s Disease and Caregivers : A Guide for Mental Health Professionals N2 - The need for mental health support within the Parkinson’s disease (PD) community has never been greater, yet many practitioners lack the knowledge or experience to address the unique challenges associated with PD. This book serves as a practical guide for mental health professionals to assist individuals with PD and caregivers through the use of cognitive-behavioral therapy techniques, with the goal of enhancing their well-being and quality of life. The book includes a review of information about PD and mental health, and four structured group programs designed to address issues that are common in people with PD and caregivers: • Coping with stress and illness • Communicating about PD • Emotional expression in PD • Interventions for caregivers The programs presented in this book can be utilized as they are, personalized for individual use, or adapted for research protocols. Additionally, the information can serve as a valuable resource for people with PD and their family members, who can learn about PD and be introduced to evidence-based strategies that can be used conjointly with professionals to improve their experience of living with PD. KW - Parkinson-Krankheit KW - Psychotherapie KW - Kognitive Verhaltenstherapie KW - Kommunikationstraining KW - Stressbewältigung KW - Parkinson-Erkrankung KW - Parkinson’s Disease KW - Training von Patienten und Angehörigen KW - psychotherapy KW - cognitive-behavioral therapy KW - patient and caregiver education KW - psychological interventions KW - communication training KW - stress management Y1 - 2024 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-345196 SN - 978-3-95826-226-3 SN - 978-3-95826-227-0 N1 - Parallel erschienen als Druckausgabe bei Würzburg University Press, ISBN 978-3-95826-226-3, 34,90 Euro. PB - Würzburg University Press CY - Würzburg ER - TY - BOOK A1 - Schneider, Wolfgang A1 - Pressley, Michael T1 - Memory development between 2 and 20 N2 - No abstract available. KW - Kind KW - Gedächtnisleistung KW - Entwicklung KW - Jugend KW - Gedächtnisbildung Y1 - 1989 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-69977 ER - TY - BOOK A1 - Marohn, Frank T1 - On statistical information of extreme order statistics N2 - No abstract available KW - Rangstatistik KW - Extremwert KW - Statistik Y1 - 1990 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-47866 N1 - Zugl.: Siegen, Univ.-Gesamthochschule, Diss., 1990 ER - TY - BOOK A1 - Macedo, José Marcos A1 - Kölligan, Daniel A1 - Barbieri, Pedro T1 - Πολυώνυμοι - A Lexicon of the Divine Epithets in the Orphic Hymns T1 - Polyṓnymoi - A Lexicon of the Divine Epithets in the Orphic Hymns N2 - The Orphic Hymns consist of a prooemium and 87 hymns addressed to several deities in a late Orphic initiation of sorts. They were composed probably in Asia Minor during the second or third century CE. The bulk of these hymns are made up of divine epithets often linked together in chains of considerable length. The lexicon attempts to give a comprehensive account of the roughly 850 epithets, bringing together the most relevant information scattered in the scholarly literature and adding others from various sources (literary, epigraphic, lexicographic, scholia etc.) in order to provide an overview of their usage and the main details of their models. KW - Orphica KW - Hymni KW - Beiname KW - Götter KW - epithets KW - orphism KW - hymns KW - Greek language KW - Griechisch KW - Orphismus KW - Hymnen KW - Lexikon Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-220613 SN - 978-3-95826-154-9 SN - 978-3-95826-155-6 N1 - Parallel erschienen als Druckausgabe in Würzburg University Press, ISBN 978-3-95826-154-9, 28,90 EUR PB - Würzburg University Press CY - Würzburg ET - 1. Auflage ER - TY - BOOK A1 - Kartenbeck, J. A1 - Zentgraf, H. A1 - Scheer, Ulrich A1 - Franke, Werner W. T1 - The nuclear envelope in freeze-etching N2 - No abstract available KW - Anatomie Y1 - 1971 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-40534 SN - 3-540-05538-X ER - TY - BOOK A1 - Halder, Partho T1 - Identification and characterization of synaptic proteins of Drosophila melanogaster using monoclonal antibodies of the Wuerzburg Hybridoma Library T1 - Identifikation und Charakterisierung von synaptischen Proteinen von Drosophila melanogaster mit Hilfe von monoklonalen Antikörpern der Würzburger Hybridoma-Bibliothek N2 - For a large fraction of the proteins expressed in the human brain only the primary structure is known from the genome project. Proteins conserved in evolution can be studied in genetic models such as Drosophila. In this doctoral thesis monoclonal antibodies (mAbs) from the Wuerzburg Hybridoma library are produced and characterized with the aim to identify the target antigen. The mAb ab52 was found to be an IgM which recognized a cytosolic protein of Mr ~110 kDa on Western blots. The antigen was resolved by two-dimensional gel electrophoresis (2DE) as a single distinct spot. Mass spectrometric analysis of this spot revealed EPS-15 (epidermal growth factor receptor pathway substrate clone 15) to be a strong candidate. Another mAb from the library, aa2, was already found to recognize EPS-15, and comparison of the signal of both mAbs on Western blots of 1D and 2D electrophoretic separations revealed similar patterns, hence indicating that both antigens could represent the same protein. Finally absence of the wild-type signal in homozygous Eps15 mutants in a Western blot with ab52 confirmed the ab52 antigen to be EPS-15. Thus both the mAbs aa2 and ab52 recognize the Drosophila homologue of EPS-15. The mAb aa2, being an IgG, is more suitable for applications like immunoprecipitation (IP). It has already been submitted to the Developmental Studies Hybridoma Bank (DSHB) to be easily available for the entire research community. The mAb na21 was also found to be an IgM. It recognizes a membrane associated antigen of Mr ~10 kDa on Western blots. Due to the membrane associated nature of the protein, it was not possible to resolve it by 2DE and due to the IgM nature of the mAb it was not possible to enrich the antigen by IP. Preliminary attempts to biochemically purify the endogenously expressed protein from the tissue, gave 99 promising results but could not be completed due to lack of time. Thus biochemical purification of the protein seems possible in order to facilitate its identification by mass spectrometry. Several other mAbs were studied for their staining pattern on cryosections and whole mounts of Drosophila brains. However, many of these mAbs stained very few structures in the brain, which indicated that only a very limited amount of protein would be available as starting material. Because these antibodies did not produce signals on Western blots, which made it impossible to enrich the antigens by electrophoretic methods, we did not attempt their purification. However, the specific localization of these proteins makes them highly interesting and calls for their further characterization, as they may play a highly specialized role in the development and/or function of the neural circuits they are present in. The purification and identification of such low expression proteins would need novel methods of enrichment of the stained structures. N2 - Für einen Großteil der Proteine, die im menschlichen Gehirn exprimiert werden, ist lediglich die Primärstruktur aus dem Genomprojekt bekannt. Proteine, die in der Evolution konserviert wurden, können in genetischen Modellsystemen wie Drosophila untersucht werden. In dieser Doktorarbeit werden monoklonale Antikörper (mAk) aus der Würzburger Hybridoma Bibliothek produziert und charakterisiert, mit dem Ziel, die erkannten Proteine zu identifizieren. Der mAk ab52 wurde als IgM typisiert, das auf Western Blots ein zytosolisches Protein von Mr ~110 kDa erkennt. Das Antigen wurde durch zwei-dimensionale Gelelektrophorese (2DE) als einzelner Fleck aufgelöst. Massenspektrometrische Analyse dieses Flecks identifizierte dass EPS-15 (epidermal growth factor receptor pathway substrate clone 15) als viel versprechenden Kandidaten. Da für einen anderen mAk aus der Bibliothek, aa2, bereits bekannt war, dass er EPS-15 erkennt, wurden die Western-Blot-Signale der beiden Antikörper nach 1D und 2D Trennungen von Kopfhomogenat verglichen. Die Ähnlichkeit der beiden Muster deuteten darauf hin, dass beide Antigene dasselbe Protein erkennen. Das Fehlen des Wildtyp-Signals in homozygoten Eps15 Mutanten in einem Western Blot mit mAk ab52 bestätigten schließlich, dass EPS-15 das Antigen zu mAk ab52 darstellt. Demnach erkennen beide mAk, aa2 und ab52, das Drosophila Homolog zu EPS- 15. Da mAk aa2 ein IgG ist, dürfte er für Anwendungen wie Immunpräzipitation (IP) besser geeignet sein. Er wurde daher bereits bei der Developmental Studies Hybridoma Bank (DSHB) eingereicht, um ihn der ganzen Forschergemeinde leicht zugänglich zu machen. Der mAk na21 wurde ebenfalls als IgM typisiert. Er erkennt ein Membran assoziiertes Antigen von Mr ~10 kDa auf Western Blots. Aufgrund der Membranassoziierung des Proteins war es nicht möglich, es in 2DE aufzulösen und 101 da es sich um ein IgM handelt, war eine Anreicherung des Antigens mittels IP nicht erfolgreich. Vorversuche zur biochemischen Reinigung des endogenen Proteins aus Gewebe waren Erfolg versprechend, konnten aber aus Zeitmangel nicht abgeschlossen werden. Daher erscheint eine biochemische Reinigung des Proteins für eine Identifikation durch Massenspektrometrie möglich. Eine Reihe weiterer mAk wurden hinsichtlich ihrer Färbemuster auf Gefrierschnitten und in Ganzpräparaten von Drosophila Gehirnen untersucht. Allerdings färbten viele dieser mAk sehr wenige Strukturen im Gehirn, so dass nur eine sehr begrenzte Menge an Protein als Startmaterial verfügbar wäre. Da diese Antikörper keine Signale auf Western Blots produzierten und daher eine Anreicherung des Antigens durch elektrophoretische Methoden ausschlossen, wurde keine Reinigung versucht. Andererseits macht die spezifische Lokalisation dieser Proteine sie hoch interessant für eine weitere Charakterisierung, da sie eine besonders spezialisierte Rolle in der Entwicklung oder für die Funktion von neuralen Schaltkreisen, in denen sie vorkommen, spielen könnten. Die Reinigung und Identifikation solcher Proteine mit niedrigem Expressionsniveau würde neue Methoden der Anreicherung der gefärbten Strukturen erfordern. KW - synaptic proteins KW - Taufliege KW - Synapse KW - Proteine KW - Monoklonaler Antikörper KW - synaptische Proteine KW - monoklonale Antikörper KW - Drosophila melanogaster KW - monoclonal antibodies Y1 - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-270205 N1 - ursprüngliche Originalausgabe der Dissertation erschienen am 19.01.2012 unter: https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-67325 ER -