TY  - JOUR
A1  - Pinzner, Florian
A1  - Keller, Thorsten
A1  - Mut, Jürgen
A1  - Bechold, Julian
A1  - Seibel, Jürgen
A1  - Groll, Jürgen
T1  - Polyoxazolines with a vicinally double-bioactivated terminus for biomacromolecular affinity assessment
JF  - Sensors
N2  - Interactions between proteins and carbohydrates with larger biomacromolecules, e.g., lectins, are usually examined using self-assembled monolayers on target gold surfaces as a simplified model measuring setup. However, most of those measuring setups are either limited to a single substrate or do not allow for control over ligand distance and spacing. Here, we develop a synthetic strategy, consisting of a cascade of a thioesterification, native chemical ligation (NCL) and thiol-ene reaction, in order to create three-component polymer conjugates with a defined double bioactivation at the chain end. The target architecture is the vicinal attachment of two biomolecule residues to the α telechelic end point of a polymer and a thioether group at the ω chain end for fixating the conjugate to a gold sensor chip surface. As proof-of-principle studies for affinity measurements, we demonstrate the interaction between covalently bound mannose and ConA in surface acoustic wave (SAW) and surface plasmon resonance (SPR) experiments.
KW  - polyoxazolines
KW  - functionalization
KW  - lectin
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-239530
SN  - 1424-8220
VL  - 21
IS  - 9
ER  - 
TY  - JOUR
A1  - Ferger, Matthias
A1  - Berger, Sarina M.
A1  - Rauch, Florian
A1  - Schönitz, Markus
A1  - Rühe, Jessica
A1  - Krebs, Johannes
A1  - Friedrich, Alexandra
A1  - Marder, Todd B.
T1  - Synthesis of Highly Functionalizable Symmetrically and Unsymmetrically Substituted Triarylboranes from Bench-Stable Boron Precursors
JF  - Chemistry—A European Journal
N2  - A novel and convenient methodology for the one-pot synthesis of sterically congested triarylboranes by using bench-stable aryltrifluoroborates as the boron source is reported. This procedure gives systematic access to symmetrically and unsymmetrically substituted triarylboranes of the types BAr\(_{2}\)Ar’ and BArAr'Ar’’, respectively. Three unsymmetrically substituted triarylboranes as well as their iridium-catalyzed C−H borylation products are reported. These borylated triarylboranes contain one to three positions that can subsequently be orthogonally functionalized in follow-up reactions, such as Suzuki-Miyaura cross-couplings or Sonogashira couplings.
KW  - synthetic methods
KW  - boranes
KW  - borylation
KW  - chromophore
KW  - functionalization
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-256827
VL  - 27
IS  - 35
ER  -